Study On Separation And Purification Of Polyphenols From Camellia Oleifera Abel

Camellia Oleifera Abel.are rich in oil, saponin, polyphenols, polysaccharides andother active substances. Among them, plant polyphenols are a class of natural organiccompounds with antioxidant biological activity and pharmacological properties, andcan be widely used in medicine, health care, food and cosmetic industry. Therefore,extraction of polyphenols from abandoned Camellia Oleifera hull and seed cake hasimportant economic and social value.In this paper, organic solvent extraction and ultrasonic-enhanced solventextraction were used to extract polyphenols from Camellia Oleifera hull and seedcake. The purification of polyphenols extracts were conducted using macroporousadsorption resins to further improve the quality of polyphenols products.First, the optimum conditions of organic solvent extraction were obtained. Underthe condition of60%ethanol solution as the extraction solvent, extraction temperature80℃, ratio of solid to liquid1:20(m/v) and extraction time30min, the polyphenolsextraction yield from Camellia Oleifera seed meal reaches1.34%, the total phenolscontent is4.76%; After5min and40KHz ultrasonic-assisted extraction, theextraction yield is1.42%, the total phenols content is4.89%.In order to further improve the polyphenols purity, macroporous resin adsorptionwere used to purify the extracted polyphenols. The suitable resin was screened outthrough static adsorption process. The resin adsorption kinetics and thermodynamicswere also studied; The best dynamic adsorption result was achieved under thecondition of0.42mg/mL sample concentration, pH=2.02, sample solution flow rate of1BV/h,60%ethanol solution as the desorbent, the dynamic adsorption resin capacityreaches4.18mg/g, the desorption capacity is3.31mg/g and the polyphenols purity is30.46%. Based on the above research, polyphenols adsorption on mixed resin wasinvestigated. Effects of different ratio of the mixed resin on adsorption and desorptioncapacities were studied and90%D101-1and10%DM301(w/w) was selected as theoptimum mixed resin with the adsorption ratio of83.75%and desorption ratio of94.80%, and the polyphenols purity reaches53.73%after the purification; Similarly,using Camellia Oleifera hull as the materials, the adsorption ratio reaches94.89%,desorption ratio is97.65%and the polyphenols purity is up to71.73%after thepurification by90%D101-1and10%DM301(w/w) mixed resin at the optimumconditions. Additionally, the adsorption kinetics and thermodynamics of the mixed resin were investigated. The results show that the adsorption equilibrium data ofpolyphenols on the mixed resin fit Langmuir isotherm adsorption model; Calculationof adsorption enthalpy, free energy and adsorption entropy of the mixed resin furthervalidate that the adsorption of polyphenols on the mixed resin is an exothermicspontaneous physical adsorption process.