The Freash Walnut Storage And Study On The Assay Method, Purification And The Activities Of Function Of The Polyphenols Of Walnut Kernel Pellicle

The walnut (Juglans regia L.) is depart of Juglandaceae Juglans vegetation. Which is an important dry fruit and woody oil plant and is one of the world’s four nuts. The traditional consumption of walnut is given priority to with dried fruit However, the dried walnut is prone to oily metamorphism in the process of walnut storage. The fresh walnut is moisture content is35%, more delicious, lower fat and more viatmin content, is better of dired walnut. The kernel pellicle of walnut by-product is rich in polyphenols in the process of refreshment. In recent yesrs, walnut polyphenol is researched to have antioxidative, inhibitory atherosis, anti-cancer, hypolipidemic and Prevention of neural degeneration. In this paper, useing fresh walnuts to research the method of storage. To establish an assay method of determine the total polyphenol of walnut kernel pellicle of walnut. Use macroporous resin to separation and purification of polyphenol. The healthy function is detected in the animal level. The main results are as follows:1. The storage effectiveness of shell walnut, walnut kernel and no pellicle of walnut kernel is tested under different temperature conditions. Results indicate that, the sensory quality of no pellicle of walnut kernel is better than others. The peroxide value and acid value of oil of walnut is increase during storage. The fresh walnuts stored at low temperature is far better than room temperature. Overall, the best of walnut storage conditions is-20℃under vacuum freezing.2. To optimize an assay method, Folin-Ciocalteu colorimetric is used to determine the total polyphenol in walnut kernel pellicle. The results indicated that the optimum conditions for determining total polyphenol are as following:1.0mL of the sample extract polyphenol,1.0mL of20%sodium carbonate solutionand1.5mL of Folin-Ciocalteu reagent dosage, treatment at60℃for1h and60%ethanol as extract solvent. The method is simple to operate, good stability, high recovery, high precis ion, reliable measurement results. It could be use as an appropriate method to measure the polyphenols in walnut kernel pellicle. The distribution of polyphenol in walnut kernel pellicle is21.73%.3. In this paper, five macroporous resins are selected to compare their adsorption and desorption performances for polyphenols from water extract of kernel pellicle of walnut. The best performance resin is NKA-9. The excellent technological parameter are:concentration of sample1.0mg/mL, current velocity1.0mL/min, the pH value of sample4.0, and detached80%ethanol. This method have high adsorption rate and less time to depurate polyphenol. The purity of polyphenols is34.6%in the crude extract from walnut kernel pellicle, increasing to69.7%after purification with the NKA-9resin. Through comparative analysis the relative peak area of HPLC, the purified polyphenols have no component loss compared with crude extract. Therefore, the resin of NKA-9is fit for the purification of the crude extract of polyphenols.4. In order to determine the polyphenol of kernel pellicle of walnut have health function by using mice to experiment. Record the body weight and food intake of mice to test the effect of polyphenol on growth. It is necessary to test the learning and memory through Morris water maze (MWM) task. The superoxide dismutase SOD activity and malondialdehyde (MDA) content are determined the antioxidant capacity of polyphenol in mice. Results indicate that walnut polyphenol is a bioactive substance, which exhibit a multiplicity of inhibit the growth of body weight and cognitive function and antioxidant system in the growth process of mice.