| To explore the useful value of plant resources is always regarded as a key point by many researchers. Sabina Przewalskii is specific tree species in our country, having lots of good features, such as highly adaptability, highly stress resistance, cold resistance, drought resistance and saline-alkaline resistance. The trees contain essential oil, and have good materials, besides that their growth area is wide, so they have high economic value. The study includes the following several aspects on Qinghai province’s S. Przewalskii, providing theoretical foundation for protecting, exploiting and scientific utilizing the species resource.1, extracted S. przewalskii’s volatile oil growing up in Qilian country of Qinghai province by ultrasonic-assisted water distillation, and the optimum extraction conditions of this method and the volatile oil’s physicochemical property are also studied. It is concluded that the best extraction technology conditions for:raw material to liquid ratio of1:10, extraction time4h, ultrasonic power500W, ultrasonic time15min. The volatile oil is light yellow and clear oily liquid, which has intense cypress aroma. Its relative density is between0.850^-0.872, and the refractive index is between1.4756-1.4789, and the specific rotation is within-44.01°～-29.65°.2, extracted, determined and identified the chemical composition and content of volatile oil from Qilian and Huzhu country, and explored these response to the change of altitude. The study shows that:(1) A total of48different compounds in the volatile oils are identified form S. przewalskii’s leaves harvested from Huzhu country’s3050,3100,3200and3400m four altitude.29,27,30and25compounds in the volatile oils from the four different altitude are identified, respectively, which percentage compositions are separately85.68%,84.53%,92.59%and84.22%.13compounds are common in the four volatile oils, which are respectively.alpha.-Pinene, Bicyclo[3.1.0]hex-2-ene,4-methyl-1-(1-methylethyl)-, Limonene,1,4-Cyclohexadiene,1-methyl-4-(1-methylethyl)-,Cyclohexene,1-methyl-4-(1-methylethy liden-e)-,1,6-Octadien-3-ol,3,7-dimethyl-,3-Cyclohexen-1-ol,4-methyl-1-(1-methylethyl)-, Cycloh-exane,1-ethenyl-1-methyl-2,4-bis(1-methylethenyl)-,[1S-(1.alpha.,2.beta.,4.beta.)]-, D1,6-Cyc-lodecadiene,1-methy1-5-methylene-8-(1-methylethy1)-,[s-(E,E)]-,Naphthalene,l,2,4a,5,6,8a-hexahydro-4,7-dimethyl-1-(1-methylethyl)-,(1.alpha.,4a.alpha.,8a.alpha.),Naphthalene,1,2,3,5,6,8a-hexahydro-4,7-dimethyl-1-(1-methylethyl)-,(1S-cis)-, Cyclohexanemethanol,4-ethenyl-.alpha.,.alpha.,4-trimethyl-3-(1-methylethenyl)-,[1R-(1.alpha.,3.alpha.,4.beta.)]-and Cedrol.(2) A total of49different constitutions in the volatile oils are identified form S. przewalskii’s leaves harvested from Qilian country’s2800、2900、3050、3100and3200m five altitude.23,28,25,25and22compounds in the volatile oils from the five different altitude were identified, respectively, and their percentage compositions are separately91.85%,95.28%,87.17%,86.48%and85.03%. The common seven constituents among the five volatile oils are.alpha.-Pinene,1,4-Cyclohexadiene,l-methyl-4-(1-methylethyl)-,3-Cycohexen-l-ol,4-methyl-1-(1-methylethyl)-,[s-(E,E)]-1-methyl-5-methylene-8-(1-methylethyl)-1,6-Cyclodecadi ene,(1S-cis)-1,2,3,5,6,8a-hexahydro-4,7-dimethyl-l-(1-methylethyl)-Naphthalene, Cyclohexa nemethanol,4-ethenyl-.alpha.,.alpha.,4-trimethyl-3-(1-methylethenyl)-,[1R-(1.alpha.,3.alpha.,4.beta.)]-and Cedrol.(3) The identified components are represented mainly by monoterpenes, sesquiterpenes and their oxygenous derivatives. The relation between altitude and the content of various class compounds is analyzed. The variation of compositions and these contents of S. przewalskii’s volatile oils are regularly affected by the ecological environment of the different altitudes.3, extracted, analyzed and identified volatile compounds from S. przewalskii’s bark by water distillation and soxhlet extraction,which growing up in Qilian country of Qinghai province. The results show that:(1)45components identified from the bark by water distillation with dissolved by diethyl ether account for88.5%of the total peak area. The main chemical components are propoxy-8-Cedrane, Bicyclo[3.1.0]hex-2-ene,4-methyl-l-(1-methylethyl)-,1R-a-Pinene,trans-Totarol,1,4-Methano-1H-indene,octahydro-1,7a-dimethyl-4-(1-methylethenyl)-,[1S-(1.alpha.,3a.beta.,4.alpha.,7a.beta.)]-,.tau.-Muurolol,1H-Cyclopropa[a]n aphthalene,1a,2,3,5,6,7,7a,7b-octahydro-1,1,7,7a-tetramethyl-,[1aR-(1a.alp-ha.,7.alpha.,7a.alph a.,7b.alpha.)-,alpha-Funebrene,Naphthalene,1,2,3,5,6,8a-hexahydro-4,7-dimethyl-1-(1-methyle thyl)-,(1S-cis)-, and so on. When the solve-nt is petroleum ether,42volatile components are identified, which account for88.2%of the total peak area. In this case the main chemical components are Cedrol, trans-Totarol, Naphthalene,1,2,3,5,6,8a-hexahydro-4,7-dimethyl-1-(1-methylethyl)-,(1S-cis)-,alpha-Funebr-ene,1,4-Methano-1H-indene,octahydro-1,7a-dimethyl-4-(1-methylethenyl)-,[1S-(1.alpha.,3a.beta.,4.aIpha.,7a.beta.)],Bicyclo[4.4.0]dec-l-ene,2-isoprop yl-5-methyl-9-methylene-, et al.23mutual components in the two results, but the relative content of these are different.(2)40components are identified from the bark by soxhlet extraction accounting for65.76%of the total peak area, which main chemical ingredients are trans-Totarol, Ferruginol,2(1H)-3,4,4a,9,10,10a-hexahydro-7-hydroxy-1,1,4a-trimethyl-8-(1-methylethyl)-(4aS-trans)-phenant-hrenone,8-propoxy-cedrane,Sugiol,4-methylene-2,8,8-trimet hyl-2-vinyl-bicycle[5.2.0]nonane,1H-3a,7-Methanoazulene,2,3,4,7,8,8a-hexahydro-3,6,8,8-tetr amethyl-,[3R-(3.alpha.,3a.beta.,7.beta.,8a.alpha.)]-.4, measured physical and chemical properties of S. przewalskii’s wood growing up in Qilian country of Qinghai province. The conclusion is that S. przewalskii’s wood ring width is small, density is light, anisotropic shrinkage is samll, water-absorbing quality is general. And moisture of the wood is6.26%, which ash is1.06%and all kinds of which extract content are higher than fokienia hodginsii, beyond that cellulose content is42%, hemicellulose content is about33.6%, acid insoluble lignin content is31.9%. |
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