| Rhodiola rosea L is a perennial herb. It’s also a kind of typical and traditional medicinal plant, which has a long history of application in China. With the deepening research in its main functional components and mechanism, how to separate and extract these active substances and then purify and identify them for the purpose of laying a solid foundation for its further development and application, will become the key and difficult research today.This project adopts the Rhodiola as the research object to investigate the processes of extraction, deproteinization, decoloration and the antioxidant activity in vitro.1. The traditional water extraction and microwave pretreatment-hot water extraction have been used for extracting the crude polysaccharide from Rhodiola rosea L. As the indicator, the extraction rate shown that latter method is better, which has a higher value of practical application, that is lower requirement, easier operation, shorter microwave time, but higher extraction rate. The effect of microwave power, microwave time, solid-liquid ratio to the extraction rate of the crude polysaccharide from Rhodiola rosea L were studied, and the optimum technological parameters were obtained by the orthogonal test. The result showed that when the solid-liquid ratio is1:60, Microsoft power is510W, Microsoft time is60s, extraction time is2h, the extraction rate of the crude polysaccharide from Rhodiola rosea L could reach at14.89%.2. We study the deproteinization of the polysaccharides included in Rhodiola rosea L, mainly focusing on the removal of protein processing by Hydrochloric acid method, Three trichloroacetic acid method and Papain method of removing protein.Hydrochloric acid method and three trichloroacetic acid method have easier operation, but lower removal rate of protein and maintenance, they could only remove the free proteins. Papain method is more complicated and strict in the Reaction conditions. However through Papain method of removing protein, which is a ideal method of removing protein, most of the protein can be removed from the extract and the polysaccharide can be well remained meanwhile. While processing the removal of protein through single factors and orthogonal test, we confirm the best technological conditions of removing protein by papain. The result showed that when the amount of enzyme is2%, the time is1.5h, and the temperature is60℃, the rate of protein-removed and polysaccharide remain could reach at92.47%and81.35%.3. The Hydrogen peroxide and the activated carbon have been used to investigate the discoloration of SRP.As the indicators, the rate of decolorization and polysaccharide remain were used to compare the decoloration effect. The Hydrogen peroxide has a higher rate of decolorization, but lost to much SRP, thus the project used the activated carbon as the better one to do the further research. According to the results of the single factor test and orthogonal test, we finally confirm the best technology conditions of decolorizing the polysaccharide in Rhodiola rosea L. The result showed that when the activated carbon dosage is1%, the decolorizing temperature is60℃, the decolorizing time is40min, the decolorization effects of the activated carbon are the best, with the rate of decolorization95.46%and the the rate of polysaccharide remain68.78%.4. This project investigate the antioxidant activity in vitro of the polysaccharide in Rhodiola rosea L by using hydroxyl radicals system, superoxide anion system and polysaccharide reductive ability system. The results show that, the polysaccharide in Rhodiola rosea L has a good antioxidant activity. |
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