Study On The Nutritional Intervention On Aging And Its Mechanism Of Proanthocyanidns From Rhodiola Rosea

Rhodiola rosea L., is the crassulaceae Rhodiola perennial herb. Many studyssuggested that Rhodiola rosea L. have anti-tumor，anti-hypoxic，protect nerve cells,enhance learning memory activity etc. In the prior period, our laboratory studies haveshown that ethyl acetate phase of R.rosea ethanol extract has the very good scavengingfree radicals effect, also found that R.rosea extract contains abundant procyanidins and thecontent is3.6%. Through the preliminary separation and purification, obtained the purity is88.3%of Rhodiola rosca oligomeric proanthocyanidns (RROPCs), and carried out thecomprehensive studies in vivo and in vitro antioxidant activity. On this basis, this paperwith rhizome of Rhodiola rosea L. produced by Zhangjiakou as raw material, nutritionalintervention effect for the senility of RROPCs were studied, And from the view point ofresistance to oxidative stress explored its action mechanism, provided theoretical basis forthe development of natural, effective anti-aging function food. The main research contentsare as follows:1. The study on antioxidant mechanism and nutritional intervention effect for the fruitflies senility of RROPCs. We were using fruit flies life test to observe the effect thatRROPCs for average life and the highest life, and test biochemical indicators of female,male fruit flies after30days feeding, assessment test substances in fruit flies in vivoantioxidant activity. The results showed that the concentration of0.04%,0.12%and0.36%RROPCs have varying degrees of prolong effect in fruit flies of average life and thehighest life, the concentration of0.12%RROPCs of prolong effect is the most effective.The highest life and average life of experimental group of female fruit flies up to71d and54d respectively, while male fruit flies up to72d and55d respectively. The concentrationof0.12%and0.36%female flies SOD activity were significantly increased(P<0.05), theconcentration of0.12%and0.36%male fruit flies SOD activity were significantlyincreased(P<0.05).It showed that RROPCs have anti-aging effect for drosophilamelanogaster, and it could be a role by increasing the body’s resistance to oxidation.2. The nutritional intervention study of RROPCs on D-galactoseactose inducedoxidative stress in mice. Mice were injected subcutaneously with D-galactoseactose manufacturing oxidative stress model, and continued fed different doses of RROPCs49days; We used serum and organs of SOD, GSH-Px activity, MDA levels in liver, brainMAO activity of mice as test index to research that intervention effect of RROPCs on theD-galactoseactose induced oxidative stress senescence. The results showed that, RROPCscan significantly increase the SOD, GSH-Px activity of serum, heart, liver and brain tissuein mice, and significantly reduce the MDA content, and showed the most prominent inhigh dose group. Compared with model control group, the serum, heart, liver and braintissue SOD activity of high dose group were increased by29.5%,28.1%,62.7%and90.8%respectively, GSH-Px activity increased by67.8%,58.4%,32.1%and50.3%respectively, MDA content decreased by19.2%,30.0%,33.3%and19.2%respectively. Inaddition, the medium and high dose group significantly reduced brain MAO activity(P<0.01), decreased by22.3%and28.6%respectively; High dose group was significantlylower liver MAO activity(P<0.05), decreased by36.5%. Therefore, RROPCs on senilitycaused by D-gal induced oxidative stress has a protective effect.