Screening Strain For Radix Astragali Fermentation And The Influence Of Its Function

In combination with the modern bio-engineering fermentation technology on the basisof the zymotechnics of traditional Chinese medieine Proeessing, we utilized N-14toferment Radix Astragali, in order to improve the dissolution rate of Radix Astragali andaehieve its high efficacy, The results showed that:To screen one bacterial strains N-14with high capability to degrade cellulose andlignin from the strains kept in laboratory, Strain N-14were identified by morphological,physiological and biochemical characteristics and16S rDNA sequence. Strain N-14belonged to Bacillus methylotrophicus. the CMC activity was27.06U/mL; Lip, MnP, Lacwere13.85,10.47and14.50U/mL.Using disc diffusion method and turbidimetric method to screen the herb RadixAstragali from14kinds of Chinese herbal medicine, it can inhibited enterohemorrhageE.coli (EHEC) but proliferated N-14, Radix Astragali can fermented by N-14. Acutetoxicity test, maximization test and histopathologic slide showed that Radix Astragalifermented by N-14was the non-toxic preparation, had high security.Using organic solvent extraction method, Water decoction of Radix Astragali ascontrol group, Compared with the contents of polysaccharides, total flavonoids andsaponins during the fermentation period. The results show that, after18h, the content ofpolysaccharide in Radix Astragali increased obviously, but the total flavonoids andsaponins were reduced, the highest was40.604mg/mL, it improved66.78%than that incontrol group, infer N-14had biological transformation ability.Dependent variable was the content of polysaccharides, based on single-factorexperiments and response surface methodology, the best extraction processing parameterwas ascertained as follows: astragalus powder4g, NaCl6g, pH=7.0,121℃sterilized15min and fermentation time24h. Under these conditions, Astragalus polysaccharideextraction rate increased57.31%.To explore the pharmacological effects of Astragalus fermentation liquid of N-14, weuse the immunosuppressant of cyclophosphamide as modeling. Rabbit was usedas experimental animal. The results show that: Compared with the cyclophosphamidegroup, test group of fed Astragalus fermentation of medium effect is most obvious.Spleen index increased by43.59%, Sacculus index decreased by0.62%, phagocytic rate increased by58.87%(P<0.01); phagocytic index increased by144.26%(P<0.01), theactivity of SOD increased by17.47%(P<0.01), the content of MDA decreased by65.38%(P<0.01), T-AOC capacity increased by50.56%(P<0.01). The results showthat fermentated Astragalus can confrontated with the damage by thecyclophosphamide, itcan ipmproved nonspecific immunity and antioxidant capacity. Fermentated Astragalusachieved better results.