Research On The Degradation Of Several Dyes And Chlorophenol Pollutants With Ganoderma Laccase

Laccase (EC1.10.3.2) belong to a family protein ceruloplasmin oxidase, laccase substrates have a wide range of high catalytic activity, etc., are widely used in organic synthesis; bioassay; eliminate toxic compounds; industrial wastewater treatment; pulp biobleaching; other fields. In this paper, laccase from Ganoderma lucidum as a starting point, Ganoderma lucidum laccase production in liquid and solid product laccase optimize the production of paint and use enzymes to degrade dyes and chlorophenol pollutants studied, the main results are as follows:Laccase liquid fermentation medium by orthogonal test and optimize the culture conditions, the result is:the best combination of media content for wheat bran1%,2.5%glucose, ammonium chloride concentration of1%yeast extract cream concentration of1.5%. Conditions for the optimal combination of liquid volume75mL, inoculum size6%strain age7d, incubation time10d, after optimization activity for253.24U/mL.Solid Fermentation laccase by single factor and orthogonal test medium and culture conditions were optimized, the result is:the dregs medium laccase from Ganoderma lucidum, by comparing several different dregs Laccase, licorice dregs solid product training Laccase activity is highest. Licorice in wheat bran ratio of3:7, the temperature is29℃, the glucose content of2%,5%inoculum size, moisture content of60%under the optimum conditions for producing laccase conditions, media activity up46707.44U/g, compared to unoptimized activity increased3times before.Ganoderma Laccase use crude enzyme solution for active Chromic blue X-BR with Reactive Blue KN-R decolorization single factor experiment, bleaching suitable conditions were:pH3.5,40℃,60U/mL lucidum laccase Under200mg/L dye concentration conditions, the activity of anthraquinone dye Chromic Blue X-BR decolorization rate of about49%, KN-R Reactive Blue pH4.0,40℃,45U/mL lucidum laccase,250mg/L dye concentration under conditions of decolorization rate of about79.11%.Ganoderma Laccase use crude enzyme solution and ABTS system on the bromocresol green, methylene blue, malachite green decolorization single factor experiment, discolored suitable conditions:bromocresol green dye concentration of 55mg/L, pH5.0, ABTS in an amount of10μL, pH4.0,45U/mL,40℃,6h, decolorization ratio was83.01%, malachite green dye concentration of35mg/L, pH5.0, ABTS in an amount of100(J.L,35U/mL,60℃,4h, decolorization rate was94.36%, methylene blue dye concentration of lOmg/L, pH4.5, ABTS amount of10μL,40U/mL,40℃,2h, decolorization rate was30.22%.Ganoderma Laccase use crude enzyme solution, respectively2,4-dichloro phenol,4-chlorophenol,2-chloro-phenol single factor and orthogonal experiment results are:optimum conditions for degradation of2,4-dichlorophenol reaction time8h, temperature50℃, pH value of5, the enzyme activity60U/mL,2,4-dichloro phenol initial concentration of40mg/L, the optimal conditions for the degradation of2-chloro-phenol reaction time8h, temperature40℃, The pH5enzyme activity60U/mL,2-chloro-phenol initial concentration,30mg/L. Optimal conditions for4-chlorophenol degradation reaction time8h, temperature60℃, pH value of5, the enzyme activity60U/mL,4-chlorophenol initial concentration,30mg/L. Under experimental conditions,2,4-dichloro-phenol,4-chlorophenol,2-chloro-phenol averages were92.3%,74.2%,68.6%, through optimized, chlorophenol degradation rate has improved significantly. By infrared analysis can be seen, benzene ring structure has been destroyed, and the product has quinones generated.