Simultaneously Determine Metal Ion By Microemulsion Hplc

Determination of iron(Ⅲ),Cobalt(Ⅱ) and Nickel(Ⅱ)in environment with PAR as precolumn derivatization reagent by microemusion liquid chromatography was studied. The optimal conditions as follows: the flow rate of 1.0 mL / min, at 530nm, a ODS-2 Hypersil column(200 mm×4.6 mm)used at room temperature, the mobile phase is microemulsion consisted of CTMAB 0.6 g,n-butanol 1.3g, n-heptane 0.1g, water 100g(containing 20mmol / L Na2HPO4-KH2PO4 buffer solution)/methanol 25:75(v/v).The results show that: in the optimal precolumn derived and chromatographic conditions containing microemulsion,Fe(Ⅲ),Co(Ⅱ),Ni(Ⅱ) complexes can be completely separated within 10 min, the sensibility has respectively improved 90.38%,45.68%,32.45%.The linear rang is respectively 0.1¹.1μg/mL for Fe³⁺,0.015¹.2μg/mL for Co²⁺ and 0.02⁰.6 for Ni²⁺; the detection limit is respectively 23.42μg / L for Fe³⁺, 9.69μg / L for Co²⁺ and 10.76μg / L for Ni²⁺. The proposed method has been successfully applied to the determination of Fe,Co,Ni in water samples and traditional Chinese medicine, the relative standard deviations are 1.76% 3.96%, the recovery is among 94.5% 101.3%; compared with AAS, the two methods have no prominently difference.The influence on the structure of microemulsion through changing the water content, pH, and temperature basedon the conductivity and microemulsion structure theory, combined with chromatographic separation conditions on chromatographic behavior of three kinds of ions was further explained.