Determination Of Benzimidazoles Residues In Foodstuffs By Hplc-ms/ms

One-step extraction and high performance liquid chromatography(HPLC)-MS/MS have been developed and validated for the determination of 6 kinds of benzimidazoles (Carbendazim, Thiabendazole, Fuberidazole, Thiophanate, Thiophanate methyl, and Imazalil)residues in food matrices including rice, corn ,bean, orange, apple, potato, spinach, fish, pork, pig liver, milk, graph, and tea.The extraction efficiency of ethyl acetate and acetonitrile,different cleanup methods including Cl8 solid phase extraction(SPE)cartridge , hydrophilic—lipophilic balance(HLB)SPE cartridge,activated carbon,florisil and Vario GPC and separation by HPLC are extensively investigated.The extraction recoveries of the seven kinds of benzimidazoles are between 61.6% and 104.8% for spiked level of 5-25μg/kg.The limits of quantification are 0.005 mg/kg for the 6 pesticides(S/N=1 0).There are good linear correlations between the peak areas and concentrations of the seven kinds of benzimidazoles,the line arrange is 0.2—10μg/mL and the correlation coefficients are above 0.999.