The Immobilized Yeast Cell Carrier Preparation And The Removal Effect Of Phenol In Waste Water

Through the microscope observation of the apparent characteristics of the sodium alginate, polyvinyl alcohol, sodium alginate and polyvinyl alcohol, the sodium alginate and polyvinyl alcohol was selected as the embedding material to preparate the immobilized yeast and the engineering bacteria immobilized yeast. According to the embedding immobilized material conditions (mass transfer properties, mechanical strength and ability of phenol, repeating usability), the optimal embedding condition was determined as:polyvinyl alcohol,3%; sodium alginate, 5%.Taken the initial concentration, the temperature and the pH value of phenol in wastewater as the control factor, a large number of experiments on effects of phenol degradation in the waste water by immobilized yeast and yeast engineering bacteria under different conditions were conducted. The results indicate that, the ability of phenol degradation of the engineering bacteria immobilized yeast was increase first and decrease then with the three control factors mentioned above. It means that, the immobilized yeast engineering bacteria for phenol in waste water to remove exist a maximum active condition. Based upon the analysis of experimental results, the maximum active condition, under which we can obtain the fastest velocity of phenol degradation, is that:pH of 6, temperature of 25℃ and the concentration of 1800 mg/L. It was also found that, the degradation of phenol was increase with a exponential growth law over time under the optimal condition.The analysis of phenol degradation in waste water by engineering bacteria and immobilized yeast immobilized yeast under different control factor (initial concentration, environment temperature, pH) were carried out. It was draw a conclusion that in the same conditions the immobilized yeast engineering bacteria for the removal rate of phenol in wastewater was greater than the immobilized yeast for the removal rate of phenol. And when the condition changes, the engineering bacteria immobilized yeast has stronger adaptability.The experiments of phenol degradation dynamic were conducted. Through the analysis of engineering bacteria immobilized yeast degradation process of phenol, the degradation process of phenol conforms to the Haldane model. Based upon the Haldane model, the phenol degradation dynamics equation was obtained by data fitting. The applicability of the phenol degradation dynamics equation was proved by other experiments data.The above waste water to remove strains of choice, And immobilized yeast engineering bacteria of removing phenol from the practical application in the waste water environmental factors control and at different time after removal of prediction of residual concentration of phenol in wastewater has certain reference significance.