The Fundamental Research On Enantioseparation Ability Of Cationic β-cyclodextrins In Capillary Electrophoresis

Most clinical drugs are single enantionmers. How to prepare and analyse chiral drugs has become the focus in medicine and analytical chemistry field. As we all know, β-cyclodextrin (β-CD) and their derivatives are being widely used for chiral selectors for durg chiral separation using capillary electrophoresis. The design and structure optimization of CD-based derivatives is very significant in capillary electrophoresis.The thesis is composed of two research sections as listed below.1. The synthesis of eight single positively charged P-CDs, including three unreported P-CDs in the litemature. All of them were successfully used to separate chiral drugs in capillary electrophoresis. The experiment discussesed and analysed the eight β-CDs’selectivity and separation ability in capillary electrophoresis. The length of β-CDs’alkyl chain and numbers of hydroxyl group (or methoxyl group) in the sidechains are found to paly important role on their chiral resolution ability. The results show that 6A-(3-hydroxy propyl)-1-ammonium-β-CD chlorine salt and 6A-(3-methoxy propyl)-1-ammonium P-CD chlorine salt have the best chiralselectivity and separation ability. The hydrogen bonding enhancement effect by hydroxyl or the methoxy could be helpful to increase of capability CDs separation ability.2. The click synthesis of 6A-4-(2-hydroxy ethyl)-1,2,3-trizolyl-6c-(3-methoxy propyl)-1-ammonium-β-CD chlorine salt. The as-prepared cationic CD was further successfully employed for the enantioseparation of～20 racemic compounds in CE. The effect of applied voltage, pH value and CD concentration on CD’s resolution ability were investigated. The good chiral recognition and separation of this new clicked cationic β-CD derivative is demonstrated.