The Synthesis And Evaluation Of Chitosan-based Protein A Immune Adsorbent For Whole Blood Purification

China have 10 million patients who are suffering from autoimmune diseases. Currently, this type of disease is still a lack of effective drug treatment method, because the cause is unclear. It is recognized as the most effective method to treat the disease with Protein A immune column. Whole blood perfusion does not require plasma separation, and whole blood perfusion avoids the possibility of infecting a variety of new viral diseases due to the large number of the inputs of new plasma. Because of the simple, fast and efficient removal of toxin molecules in the blood, the application of whole blood perfusion is more and more extensive and the treatment of the disease has expanded from drug poisoning and liver and kidney disease to a series of immune diseases. At present, the most commonly used Protein A immune columns are Immunosor Protein A immune column of Sweden Campbell and Prosorba Protein A immune column. They both received the certification of the U.S. Food and Drug Administration (FDA), and are widely used in the treatment of autoimmune disease. However, they also have some problems, such as poor mechanical strength, ligant easy to fall off, large flow resistance, slow purification speed, high price and so on.Chitosan has good biocompatibility and non-toxic to human body. A large amount of hydroxyl and amino groups on the surface makes it easier to modify. At the same time, chitosan can be spun into fibers, blood flow along the fibers can greatly reduce resistance of whole blood perfusion.In this paper, chitosan fibers wre selected as the matrix material. A good synthetic path of protein A immune adsorbent was established by epoxy activation method, When EPI activation time was 4 h, the amount of EPI was 1.5 mol/L, the final concentration of NaOH was 0.4 mol/L, the amount of DMSO was 0.5 mol/L, the reaction time of heparin connection was 12 h, the amount of heparin was 10 mg/mL and it had an appropriate length of connecting arms, A chitosan-based protein A immunoadsorbent with good blood compatibility wouldl be obtained. IgG adsorption capacity could reach up to 84 mg/g.Considering the internal pressure of the perfusion device, the adsorption of IgG and hemolysis rate, a better whole blood perfusion pattern of Protein A grafted chitosan fibers wae chosen. The adsorption of non-antibody component in plasma was not significant.