| Synthetic colorant is an important part of food additives. Overspray and over limited using synthetic colorants have become an increasingly serious problem. Now,there are only five national standards for the detection of water soluble synthetic colorants. There are only a few synthetic colorants detected and the application scope is narrow. This situation has brought considerable difficulty to the government supervision and law enforcement. The lack of standards and regulation as well as the limited detection methods lead to a blind area, resulting in an ineffective protection of the people’s health. The existing national standard can’t meet the demand of different kinds food testing. To establish an analytical method that can detect multicomponent synthetic colorants of complex food has important significance.In this work, a research project based on the regulatory requirements is carried out by summarizing the existing national standard and the literature, in which the advantages and disadvantages are analyzed. We have established a detection method of the quinoline yellow and other synthetic colorants in food which based on polyamide powder adsorption principle. The method use ethanol ammonia solution to extract water soluble synthetic colorants, hexane get rid of fat and polyamide powder to purify and enrich. HPLC-DAD is used for qualitative and quantitative analyses. The method has low matrix interference, high rate of recovery and accuracy.We have also established a detection method of indigo and erythrosine in foods with solid-phase extraction coupled with high performance liquid chromatography(HPLC). In the method, we used ethanol ammonia solution to extract water soluble synthetic colorants, hexane to get rid of fat, and WAX solid phase extraction column to purify and enrich the sample. This method has solved the problem and difficulty to extract indigo and erythrosine.In the end we established a method of LC/MS/MS for identification of individual component, for ensuring the accuracy of the data to avoid false negative and false positive samples. This research provides strong technical support for supervision and law enforcement, and also lays the foundation for establishing detection platform for analysis of colorant and dye. |
