Lily Colchicine Separation Of Applied Research

Colchicine, which is a principal tropholone alkaloid, was isolated from Colchicum autumnale Liliacease in 1 820. Much attention was paid to colchicine and its analogues for their special activities and officinal value.In this paper, an physiological component colchicines was studied in Bulb of Lily ,which was taken from longshan county , Hunan province. Researches on colchicine from the Bulbs of Lily abroad were reviewed, including resources of colchicine, methods of extraction, methods of separation and characterization.Extraction of colchicine from the bulbs of lily was studied by supercritical carbon dioxide fluid and the extraction technological conditions were optimized by vertical-method. In selected technological conditions, the influence order of each factor is: extraction temprature > extraction time > extraction pressure > pre-soaker dosages.The content of colchicine in extracts was determined directly by high performance liquid chromatography (HPLC). The results showed that at 40 , 18MPa and with ethanol as pre-soaker and extract 2h.. Under the given conditions, colchicine concentration increased greatly from 0.049% in natural sample to 6.4% in extract with supercritical fluid extraction(SFE). The comparison of SFE and organic solvent extraction suggested that theformer is a quick, safe, non-toxic and environmental friendly method with higher yield and selectivity. So supercritical fluid extraction of colchicine from the bulbs of lily is practicable.Pure colchicine was isolation from the extracts through thin-layer chromatography and it was identified by using spectral techniques such as1HNMR, IR.Meanwhile, Rearch on effect of lilium extract for scavenging hydroxyl radical(OH), It can be applied to develop healing ointments of lily.