The Tetramethylpyrazine Extraction Purification And Stability Studies

Chuanxiong zine (or tetramethylpyrazine) is the rhizoma of chuanxiong hort that belongs to the family of umbellar plants. Clinical research shows that the chuanxiong zine is an effective medicine to deal with the cranial cerebrovascular disease by increasing the blood flux of coronary artery, decreasing blood viscosity and improving erythrocyte transfiguration as well as restraining the biological synthesis of thrombin A₂(TX A₂) and alleviating the damage of brain tissue resulted by hyphemia. In addition, Chuanxiong zine can also be used to remedy neurotrosis and some diseases occurred in lung and kidney.The macroporous adsorption resin is a kind of functional macromolecule materials, which is produced through the polyreaction using polymonomer, glue, hole-formed additives, dispersant and other additives. Its physical and chemical property is very stable and it does not dissolve to acid, alkali and organic solvents. Furthermore, it can selectively adsorb the organics from the aqueous solution by physical adsorption.This study was to evaluate the performance of separation/purification of Chuanxiong zine from the lab-prepared standard aqueous solution using various macroporous resins. Results showed that the resin of AB-8 had the best property to separate the Chuanxiong zine from aqueous solution. During the experiments, the extraction and resin pretreated conditions have been optimized. In addition, the factors of affecting AB-8 resin's performance of separation/purification of Chuanxiong zine were also investigated in this study, which included the specific adsorbed amounts of sample (adsorbed amounts of Chuanxiong zine/amounts of resin loaded in the column), time...