Thick The Humoral Film Split Of Chiral Drugs And Their Dynamics

Chiral technologies were reviewed in detail in this paper, and the resolution ofclenbuterol(Cle) by bulk liquid membrane (BLM) were further investigated. Amethod for capillary electrophoretic enantiomeric resolution of racemic clenbuterolhas been established with HP-β-CD as the chiral selector. General equations anddata analysis approach are presented to relate molibilities to equalibrium constantsin simple binding equilibria and used to determine binding constants andthermodynamic parameters for host-guest complexation of clenbuterol enantiomerswith HP-β-CD. The effects of type and concentration ofβ-CDs, buffer type and itsconcentration, pH of buffer, separation voltage and capillary temperature wereinvestigated in detail, respectively. The appropriate conditions was: 30 mmol/LHP-β-CD, 50 mmol/L phosphate solution (pH 2.5) as running buffer, separationvoltage in 24 kV, and operation temperature Of 20℃, the baselines separation couldbe obtained with resolution of 6.78. The quantitative ability of capillary zoneelectrophoresis also be researched.The distribution behavior of clenbuterol enantiomers was examined in theaqueous and organic phase containing combinatorial chiral selectors:Dibenzoyl-D-tartaric acid (D-(+)-DBTA) and Di-p-toluoyl-D-tartaric acid (D-(+)-DTTA), in order to establish the optima chiral extraction conditions in thisexperiment. The influences of the molar concentration ratios of combinatorial chiralselectors, organic solvents, lipophilic anions and pH in aqueous phase, temperatureon chiral extraction performance were investigated, and the thermodynamic.parameters for chiral extraction procedure were also determined. It is shown that theenantiomer excess (e.e.％) can be above 10% under the optima chiral extractionconditions.Bulk liquid membrane containing chiral carrier was used for resolution ofracemic clenbuterol, the optimal chiral separation conditions and the kineticresolution model were established in this experiment. The influences of the molarconcentration ratio of chiral carrier and pH in aqueous phase on performances ofchiral resolution were investigated. The chiral resolution of bulk liquid membranewas analysed by means of a kinetic model involving two consecutive irreversiblefirst order reactions. The pseudo-first order apparent rate constants, k₁ or k₂ ofinterfacial reactions in membrane-feed solution interface or membrane-strippingsolution interface were determined. The results show that the separation factor is above 1.08 under the conditions of pH 7 in aqueous phase with the molarconcentration ratio of 1: 4, and the kinetic resolution of clenbuterol can beevaluated by two consecutive irreversible first order reactions under the optimalconditions.