Nanotechnology Applications In Rna Research

Since the introduction of the concept of nano technology by R. Feynman in 1959, it has been paid great attention and developed rapidly. The development of the nano technology drove the development of some other subjects and brought some new interdisciplines. Nowadays, nano biotechnology is the research of the world, and which has been developed greatly. This paper is focused on the application of the nano-technology in the field of the research of the RNA.1. The magnetic nanoparticles were synthesized by the chemical co-precipitation. And the magnetic nanoparticles were coated the silica in the reverse microemulsion. The surface of the nanoparticles was modified with active thiol. Then the beads were connected with oligo(dT) probe. The functionalized magnetic nanoparticles were used as the general agent to separate the mRNA from K562 cell. The results showed that the functionalized magnetic nanoparticles separated the mRNA from K562 cell effectively, which offered a valuable tool to the research of the mRNA.2. A single K562 cell was achieved from the massive cultural solution through semi-quantitive technique, tne selective lysis NP-40 was used to crack the membrane. Then the mRNA was separated from the lysate with the oligo(dT)-modified nanoparticles. Two step RT-PCR method was performed with the solid phase reverse transcription. The result of the agarose gel electrophoresis shows the mRNA was extracted from the single K562 cell and was amplified successfully.3. Based on the theory of the nano technology, the magnetic orientation composite particles were synthesized through the method of complex coacervation. The results show that the composite particles have good response to the magnetic field and high encapsulation efficiency to the siRNA. The composite particles have the effective function of inducing apoptosis on K562 cells.