Gentamicin Produce Antibacterial Activity Of Group Sub-ant-a And The Ant-b, A Preliminary Study Of The Fermentation Liquid

Aminoglycoside antibiotics has occupied fairly large market share, it always used on clinical as anti-infective drugs because of its wild antibacterial spectrum, fine curative effect, stable property and any other advantages. The minor component produced durining the fermentation process which has the potential to be some new drugs with high activity and low side effect. Meanwhile, we`ll screening more new activity compounds if we do further research on minor component and metabolic mechanism of the producing strain.In this paper, we validated that Micromonospora purpurea SIPI-A-2020 can produce Gentamicin by strain cultivation, fermentation and extracted Gentamicin from the fermentation broth. Furthermore, we use Bacillus subtilis as screeing model to screening secondary antibiotic activity compounds from the fermentation broth. As a result, we got ANT-A and ANT-B two active components. ANT-A mostly resides in the mycelium and dissolved in the methanol. The extraction process including desalt and decolor from the mycelium methanol soak, and using silica gel column partition chromatographic separation, finally through crystallize and precipitation got 96% purity compounds by TLC and HPLC validated. ANT-A has special character absorption wavelength on DAD-UV detecion.We got its molecular weight is 315 by MS spectra.The chemical structure was analyzed by 1H-NMR.We proposed that ANT-A is an pseud-disaccharide compouds with unsaturated double bond. ANT-B is water soluted with higher polarity than Gentamycin. It has high antibacterial activity against G+ and G- bacterial. We use macropore Lewatit resin D301for decolour and then the decoloured solution was loaded on a column of HD-2 macropore cation exchange resin.As for the adsorbed ANT-B was eluted with 0.125% ammonia water by us. After using activated carbon for secondary decolour and CM carboxymethyl cellulose resin for depurate, we can got comparatively purified compounds, at last. We proposed that ANT-B` s molecular weight is 444 by MS spectra. Through our research we got two active compounds ANT-A and ANT-B which has not been reported before.The two compounds may be developed into any new drugs if we do further research on them.On the other hand, we proposed that there will be anyother metabolic pathway in Micromonospora purpurea.