Organisms, The Internal Structure Of Three-dimensional Microscopic Imaging Technique

Three-dimensional reconstruction of microscopic images (3DRMI) of internal structure of biological specimens is one of the most important research fields in biological engineering. It has achieved great successes in the world over last decade. However,in our country,the research of this technology is on the primary state. The technology of 3DRMI involves optics,mechanics,electronics and computer science. The procedure of 3DRMI includes the steps as follows:firstly,make section preparation of the biological sample;secondly,capture the serial images of the section plan and save them in sequence;thirdly,process the serial images;finally,reconstruct three-dimensional model of the serial images. Using 3DRMI system,we can get the data and three-dimensional inner or outer structure of any organ. Therefore,the research of 3DRMI is of great value. This thesis discusses the basic principle and the method of 3DRMI technology,introduces the 3DRMI system that we have built,and focuses on the research of the three-dimensional reconstruction method of serial slice images. In the final part,we carry out the experiment on the 3DRMI system and present the result.The thesis is organized as follows:Chapter 1 introduces the history and development of several internal imaging methods. This chapter ends with the depiction of the primary research objectives of the thesis.In Chapter 2,we discuss the essential principle and feature-based design of 3DRMI imaging system. The basic concepts and methods of the slice,capture and reconstruction technology closing to the 3DRMI are expounded.Collection-technology on capturing a serial of slice image is introduced in Chapter 3. This part includes microscopic imaging system,lighting system,the hardware and software of capture system.The prepare-technique of the serial sample slice is discussed in Chapter 4,including the design of the slice machine,the software and hardware of the step-motor driving system. The technique of preparing the biological sample is discussed in this chapter.Chapter 5 presents the three-dimensional reconstruction method of a serial slice images. This chapter is organized as following:firstly,introduce several 3D reconstruction methods;secondly,design the software of two-dimensional image processing;thirdly,analyze the algorithms of the three-dimensional reconstruction in detail and discuss the design and implementation of our serial image-reconstruction software system;finally,present the other reconstruction method and its development.Chapter 6 introduces the 3DRMI experiment system and provides the corresponding experiment results. In this chapter,we simulate the 3D reconstruction of three serial images and give the reconstruction model of three serial actual slices images.The final chapter summarizes the main results and makes conclusions of the thesis. Some future research work is also described.