Epidermal Growth Factor Mrna Expression In Pigs In The Female Genital

Epidennal growth fector (EGF) has the rule of stimulating the proliferation of epithelial cells and a variety of cell types. It is a important factor to the growth and differentiation of cells. It plays important roles in the course of female mammalian reproduction, including foliicu]ar development, oocyte maturation, zygote formation, early embryo development, embryo implantation and uterus degeneration, pregnancy maintenance, pamnhition, and so on.. So rnaldng finlher known about the laws of EGF changes to the reproduction cycle in female pig reproductive organs, ovary, oviduct and uterus, is very well to mammalian oocytes maturation and fertilization hi vfim, embryo development in vitro and embryo transplantation, and well to curing the disease of the female reproductive system. To study the role of EGF mRNA hi female reproductive organ of pig, reverse transcription- polymerase chain reaction (RT-PCR) was used to examine the expression of EGF mRNA in the ovary oviduct and uterus. We detected that EGF mRNA was expressed sflungly lii the oocyte, and is also found hi gmnulosa cells, the cell fium smaller foflicular expressed stronger than fium bigger one. In the corpus hemonbaglcwn corpus luteurn, lean type and pseudocorpus-luteum, EGF rnRNA was detected,, no distinct difference can be seen in them. The EGF mRNA expressed strongly in fimbria end, ampulla and isthmus of oviduct, in the big follicular stage, ovulation stage, pregnancy stage and spurius pregnancy stage, we can not see any distinct change in them, but hi the medium follicu]ar stage,it is weaker. hi the uterus, is the same as the oviduct So we guess that EGF mRNA in oviduct and uterus may be controlled express or there is other way in regulating the EGF content in diem, except in the medium follicu]ar stage. We found that when the concentration of dNTP and Mgcl2 was 1mM and 2.5mM in reverse transcription also, it is well for them to be 0.3mM and 0.75mM in PCR, if the concentration of Mgcl2 is or over 1.0mM in PCR, the non-peculiar band will be fouriL 5% glycerine and dimethyl sulfoxide can improve the PCR by lower die temperature of untie and separate the DNA chain...