He-ne Laser Mutagenesis Mushrooms Protoplast Breeding Fast-growing High-yield Strains And Mushroom Liquid Submerged Fermentation Process,

In this paper, with laser-irradiation and protoplast technique, the mutants were screened whose mycelial growth speed was increased. We studied the submerged cultivation of the mutants, offering the base of modern fermation technique.The result showed that protoplasts were easier to be obtained when Lentinus edodes were cultured to the mid-later exponential stage, while the mycelium co-treated with 2% Lywallzyme, at 32. And the regeneration frequencies reached to 3.24% on hyperosmotic medium containing 0.6M MgSCXHe-Ne laser (A =632.8nm) irradiated protoplasts for different time. The analysis of esterase isoenzyme indicated that the mutants' esterase is-enzyme patterns were of great difference from the starting strain. Solid culture, liquid culture told the mutants' mycelial growth speed and biomass were different from starting strain too. So it was concluded the mutants genetic substances had changed and different doses had different effect on protoplasts. Laser is a good mutagen.From the industrial angle, we studied the submerged cultivation of the mutants; discussing the law of a series of parameters, for example, reducing sugar, amino nitrogen, pH with the change of mycelia growth in the course of activation. After studied the effect on wet mycelial amount of the different medium, different inoculation methods, we got the best fermentative media and fermentative procedure.