He-ne Laser Mutagenesis Agaricus Blazei Protoplast Breeding Fast-growing High-yield Strains And Agaricus Blazei Submerged Fermentation Process Research

In this paper, with laser-irradiation and protoplast technique, the mutants were screened whose mycelia growth speed was increased. I studied the best fermentative procedure conditions of the mutants that were cultivated in submerged culture medium, and that will offer the base of producing industrially Agaricus blazei Murrill submerged strains.The results showed that the best conditions of Agaricus blazei Murrill protoplast production and regeneration were to use the strains after cultivated in submerged culture medium for four days, while the mycelia co-treated with 2% Lywallzyme, at 32癈, for five hours, and the production rate of protoplast reached to 9.08million per milliliter, and the regeneration frequencies reached to 2.50% on hyperosmotic medium containing 0.6 M MgSO4.He-Ne laser (r=632.8nm) irradiated protoplasts for different time. The analysis of esterase isoenzgme indicated that the mutants' esterase isenzyme patterns and Rf were of difference from the mycelidium. After solid culture and liquid culture, we can see that mutants' mycelial growth speed and biomass were different from mycelidium. So it was concluded that the mutants' genetic substances had changed and different doses had different effects on protoplasts. Laser is a good mutagen for Agaricus blazei Murrill.After studied the effect on wet mycelia amount of different medium, different inoculation methods, we got the best fermentative media and fermentative procedure. And we also got the law of a series of parameters about the mutants when submerged cultivated, including reducible sugar, amino nitrogen and pH.