Eggplant Resistance To Verticillium Wilt Of Verticillium Wilt Mutants In Vitro Screening

Verticillium -wilt is one of the main diseases of eggplant. Shorting of excellent resistant resources, it is slowly getting on in the resistance breeding field of eggplant to resist to Verticillium -wilt. By using the cell engineering technology, in vitro selection for resistant mutant will provide excellent resistant materials in the diseases resistant breeding of eggplant, therefore it is significant in theory and practice.In this experiment, the stem apexes of eggplant variety,SANYEAQUE, were served as experiment material treated with Co60-/ - rays,NaN3,EMS from 1998 to 2002, and the main experiment results showed:1. The better medium (PDA) for producing toxin, the method to inject toxin, and the selection pressure of toxin have been determined.2. The technology system for selection eggplant mutant to resistant to Verticillium wilt in vitro culture has been established.3. The mutant to resistant to Verticillium wilt of eggplant has been obtained, and designed as 142-E.The level of resistant to Verticillium wilt of eggplant is better than that of control, SNAYEAQUE.4. The obvious difference appeared between obtained mutant and wild control SANYEAQUE, when making a comparison of electrolyte permeability of host, the activity of superoxide dismutase(SOD) and peroxidase(POD) and esterase isoenzymes,thus indicated that the level of mutant to resistant to Verticillium wilt of eggplant has developed in some extends.5. The characteristic of mutant 142-E has few changed except the shape of leaf, comparing with that of the control SANYEAQUE.On the other hand, the analysis of cytology(chromosomes) indicated that the type of cell core is 2A of Stebbins, and the formula is 2n=2x=24=22m+2sm(SAT), as same as those of control SANUEAQUE. All of these showed that the genetic of regenerated plants of mutant 142-E is steady.