Uv-b Radiation Of Co <sub> 2 </ Sub> Laser On The The Gentiana Physiology, Growth And Active Ingredient And Its Processing And Storage Methods Of Evaluation

To improve the industrialization of Gentiana macrophylla Pall, in shaanxi province, we studied separately: 1, Effects of CO2 laser irradiation on seed germination and seedling growth of Gentiana macrophylla Pall.; 2, Impacts of appropriate supplement of UV-B radiation on physiology, growth and quality of Gentiana macrophylla Pall, under field condition; 3, the methods of processing and store of Gentiana macrophylla Pall.The dose of CO2 laser was 3.2 mw.mm-2, the treat time was from Os to 120s; UV-B supplementary dose was 2.18 kJ.m-2.d-1 and 3.36 kJ.m-2.d-1 respectively from 9:00 to 17:00 everyday during growing season (from May to Oct); The processing methods included microwave-drying, drying, braising , insolation , steaming and airing, the temperature condition stored had also been studied.The results showed that: (1) CO2 laser irradiation of lower dose (30s - 60s) promoted seed germination, Improved content of total chlorophyll , chlorophyll a , chlorophyll b and soluble protein, and then promoted the seedling growth of Gentiana macrophylla Pall..(2) Supplementary UV-B radiation had a little effects on growth but positive effects on quality of biennial Gentiana macrophylla Pall. The height, root weight, total biomass, content of total chlorophyll and chlorophyll a were decreased when compared to ambient controls in growing season, but not obviously different; The growth of two treatment groups were influenced less especially with the continuance of UV-B radiation. In addition, the content of UV absorbing compounds and soluble proteinand PAL enzyme activity of the both treatments were increased as compared with controls. Those positive effects which should be defendent measures of plant to the supplementary radiation, had guaranteed the growth of Gentiana macrophylla Pall in a certain degree and had increased content of gentiopicroside. The reason of reduction of soluble sugar content may be that resource allocated more to secondary metabolism under supplementary UV-B radiation. In a word, we can promote the content of gentiopicroside in Gentiana macrophylla Pall, by supplementing properUV-B radiation.(3) The study on the processing and storage showed that microwave drying and high temperature drying were the better processing methods which can reduce gentiopicroside's loss greatly. It is a good method to store Gentiana macrophylla Pall by refrigeration after drying. Allowing for the actual conditions, the planters had better store dried Gentiana macrophylla Pall in cool and ventilated place, that is a good choice especially in autumn and winter.