Sichuan And Chongqing Local Wheat Varieties Genetic Diversity Of Storage Protein Allele

Sichuan white wheat (SWW), widely distributed throughout Sichuan Province (now Sichuan and Chongqing), is a group of Chinese endemic wheat, have many special genes and high genetic diversity. Study on the diversity of SWW is very important to the modern wheat genetics and breeding. In order to find new gene resource and provid novel materials for the wheat quality improvement, allelic variation of gliadins and high molecular weight glutenin subunits (HMW-GS) were investigated by using acid polyacrylamide gel electrophoresis (APAGE) and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of 49 landraces native in Sichuan and Chongqing. The genetic relationships among those SWW accessions were constructed by gliadin polymorphism, and HMW-GS combinations were also totally analysized. A few HMW-GS subunits were also detected in the present SWW accessions, which have not been reported on other common wheat. Relationship between the component of the wheat endosperm proteins and the genetic diversity were also disscussed. The major results were listed as following:1,The abundant variations of gliadin in 49 landraces native in Sichuan and Chongqing were detected by APAGE. A total of 39 gliadin patterns were observed. There were 18, 6, 6 and 9 allelic of gliadin patterns atω,β,αandγzones, respectively. Frequency of each pattern ranged from 2.13% to 100%, with an average of 44.8%. 13 to 22 patterns cound be identified in each accession. Among a few accessions, the gliadin patterns were similar to each other, and these accessions cound not be distinguished by APAGE.2,The genetic similarity (GS) among 49 landraces native in Sichuan and Chongqing accessions were calculated using Quantity One software. The genetic similarity varied from 0.58 to 1.000, with the average of 0.88. The gliadin diversity of those materials accessions from different geography was estimated. Furthermore, the clustering results indicated that genetic relationships among those wheat based on the gliadin were associated to its geography distributions.3,Allelic variation of high molecular weight glutenin subunits (HMW-GS) was...