Shandong Expansion Of The Streptomyces Fermentation And Antibacterial Activity Of Substance Purification Analysis

In screening process of agriculture antagonistic strains, streptomyces strain 24# was isolated from soil, which was found to be strongly antibiotic to about 30 plant pathogenic fungi, such as Helminthosporium sorokimun, fusarium graminearum Schw., Gaeumanomyces graminis, Valsa mali, etc. The secondary metabolites produced by the strain had malformation effect on these plant pathogenic fungi. The 16S rDNA sequence analysis and the traditional identification methods was used to identify the strain. Based on test results, this strain was identified as Streptomyces thermocarbonxydus var shandaensis.This article carries on the expanded fermentation experiment and the big mouse acute toxicity test and to its produces the anti- fungus active constituent to carry on the depuration and the physics and chemistry nature analysis to Streptomyces thermocarbonxydus var shandaensis. First carries on the expansion to the Shandong chain mold to ferment, according to the low cost high active principle, carries on the culture medium to optimize, determined the most suitable culture medium composition, simultaneously carries on the experimental fermentation, in the examination when ferments each target, has laid the rationale for the actual production.Passing the oversized mouse acute toxicity test, uses the law to 10000mg/kg.bw to fill the stomach contamination, after 14 days big mouse normal growth, does not have a death, belongs to the low poisonous matter according to the present agricultural chemicals standard, according to the actual Canadian type quantity, belongs to the non-toxic matter.Simultaneously carried on Streptomyces thermocarbonxydus var shandaensis to produce the anti- fungus active constituent the depuration and the physics and chemistry nature analysis. First carries on through the organic solvent extract experiment to the active constituent thickly raises, then carries on through the silica gel column chromatographic analysis except the part in removes the pigment. In used...