Starry Micropropagation Bioreactor Applications

Tissue culture is applied widely in the proliferation of Gypsophilapaniculata L., but conventional tissue culture technique still need to beimproved. Based on the confirming of medium composition of conventionalsolid culture, using bioreactor to proliferate adventitious shoots is ofimportant meaning for offering shoots largely.Medium composition of conventional solid culture was studied in thispaper using stem segment as experiment material; For improving the defectof the conventional solid tissue culture in the adventitious shootsproliferation, this paper explored the feasibility of the bioreactorculture on the rapid proliferation of Gypsophila paniculata L. and studiedseveral controllable factors affecting the proliferation during liquidculture in bioreactor.The effect of medium composition of conventional solid culture wasstudied, the results showed that the MS medium was more suitable for theproliferation than White, SH and B₅ medium; Adventitious shoots with betterquality were obtained in 2MS medium containing 20/40 mM of NH₄⁺/NO₃^- and 90mM of total nitrogen in MS medium. Compared with the fructose and glucose,sucrose was in favor of the proliferation of Gypsophila paniculata L..Furthermore, 30 g/L of sucrose was optimal for proliferation and decreasingvitrification rate of Gypsophila panlculata L.The comparision was carried out between the conventional solid cultureand bioreactor culture for the rapid proliferation of Gypsophilapaniculata. L. The results showed that during the culture period the numberof adventitious shoots from per explant reached 15.7 in the bioreactorculture, while only 11.6 shoots was observed in the solid culture,furthermore, the fresh weight and dry weight of the adventitious shootsin the bioreactor culture were significantly higher than that in the solidculture, and shorten the growth period greatly. Therefore, rapidpr(?)ation of Gypsophila paniculata L. using bioreactor is an economicaland feasible method.Inner controllable circumstance of, proliferation culture ofGypsophila paniculata L. was adjusted using 2.5 L air lift bioreactor whichwas of column shape, the results showed, that inoculation density of 35##原图像不清晰 explants was more favorable for proliferation of Gypsophila paniculataL. than that of 25 and 45 explants, we not only gained vigorous adventitiousshoots but also took advantage of the space efficiently; High lightintensity was needed for proliferation of Gypsophila paniculata L. inbioreactor, but over-intense light intensity inhabited the formation ofadventitious shoots and the increase of shoot length, 4800 Lux wasconsidered to be the most effective light intensity. It was good forp(?)feration of Gypsophila paniculata L. in bioreactor to use spargerwith small pores of 15μm and afflux air volume of 0.1 vvm at the bottom, anduse circle-shape sparger to inject air at the top of the bioreactor after4 days inoculation.