Microenvironmental Control In Bioreactor For Node Culture Of Grape

For improving traditional tissue culture system, this paper used nodes of grape '5BB'plantlet in vitro as explants to investigate several factors affecting micropropagation in air-lift bioreactors. The results showed that two bioreactor cultures were better than solid culture for shoots proliferation and growth, the raft bioreactor was more favorable than ebb and flood. During raft bioreactor culture,60 nodes of inoculation density,1600 Lux of light intensity, aeration at 0.1 wm, and 20 g·L-1 sucrose in medium were suitable when working volume was adjusted to 2 L in 5 L air-lift bioreactor.The kinetic studies of plantlet growth and pH, EC, and sugar concentration in medium were carried out. pH and EC declined with culture period, the lowest pH was observe at 20 days, after then pH increased again until to nearly initial value; at 40 days of culture, the lowest EC value was measured. Sugar content was exhausted when highest growth appeared at 40 days. Plantlets derived bioreactor cultures were transplanted into different substances, all survived on vermiculite substance while only 45% was survived on sand. Moreover, by grape stomata in leaves during transplanting, the stomata having closed function were found after fourteen days of transplanting.Using bioreactor to produce high quality plantlets of grape is a efficient way for replacing traditional tissue culture method and increasing economic benefit during large scale industrial production.