Purification And Biological Activity Of Burdock Oligosaccharide

The Arctium lappa L. is biennial plants which belong to the burdock genus of the composite, and also named eastern ocean panax or"dalizi"and so on. The burdock roots deep in soil, contains earth cresm, and has a great nutrition value and broad pharmacological activity, not only can be eaten but also as drug .This paper study the biological activity , extract and purify the oligosaccharide BOS from the burdocks roots.this experiment uses the root of the burdocks as material , taking the isolating and purifying technique, and extract saccharide in the materials. The oligosaccharide which is extracted is identified as a singlely pure substance. Using the muscovado as the beginning materials to count, the percentage of pure Burdock oligosaccharide BOS is 75.9%.the oligosaccharide content of the product is determined 98.4% by phenol-vitriol method.Using experimental rat to do experiments, the function of burdock oligosaccharide to resist oxidation and decrease blood fat is studied ,the results shows that BOS can significantly reduce TC,TG and LDL-G, minish the arteriosclerosis index, can prevent the high fat blood and oligosaccharide.besides, also it could lower the liver weight, TC and TG, so as to eliminate fattiness pathological changes.Burdock oligosaccharide out of body has no control on the stomach BGC823 cell and Lewi slung cancer cells; Burdock oligosaccharide in the body has control on cancer growth of the Mice Bearing Tumor, the control percentage amount up to 42.81% at the does of 250mg per kg. By inspecting TNF-αexchange in the blood serum of every group, found that TNF-αexchange in the blood serum appear to rise up with the dose increasing of the burdock oligosaccharide, from the above results, it is believed that the resistant function to the internal cancer is realized by increasing immunity regulating function of the body and the relative with the cancer.this experiments study effects on the immunity functions of the rats, using intraperitoneal macrophage cell as objects, under extra-cultivated conditions, using Oxygen Free Radicals which are released and activated by macrophage which are bited and stimulated as a consult. Based on the experimental results of many group, it is founded that burdock oligosaccharide has significant immunity stimulation, namely, in contrast with the blank control, can produc more NO by stimulating on intraperitoneal macrophage cell, and with the rising concentration of oligosaccharide, the stimulation strengthens, and also has a relation between quantity and effecency.