Fugu Obscurus Nature Of The Baff Gene-related Sequences Are Discussed

The research aimed to explore whether there was BlyS gene in the genome of Fugu obscurus,which exists in mammals and birds.Human B lymphocyte stimulator(also known as BAFF,BlyS,TALL-1,zTNF4,THANK)was found and considered to be one member of tumor necrosis factor(TNF)family.It was closely related to pathogenic mechanisms of immue system diseases.Low level or excessive expression of it all can induce immune imbalance.Specific primers were designed based on the reported conserved BlyS gene regions,which were from four species:Homo sapiens, Mus musculus,Sus scrofa and Gallus gallus.Ctenopharyngodon idellus and Mylopharyngodon piceus were taked as external control group.Homo sapiens were positive control.By PCR amplification,purifycation and withdraw,the products were cloned into pUCm-T vector and transformed into E.coli and sequenced.Three specific bands were obtained through PCR amplification,with the size 542,349 and 221bp. 542bp-sequence was highly conserved in Fugu obscurus and Ctenopharyngodon idellus.The A content of 542bp-sequence was 18.8%and the lowest among four kinds of bases.It belonged to "N(arbitrary nucleotide)",which weren't sequenced in Fugu rubripes genomic library(2007).Sequences with the size of 349bp and 221bp were conservative in Fugu obscurus,Fugu rubripes and Ctenopharyngodon idellus. 542,349 and 221bp were specific amplification.The comparison results between Fugu obscurus and Fugu rubripes showed that the main type of base mutation was insertion or deletion in the process of molecular evolution.Meanwhile,we translated the DNA-sequences by biological sol,are,such as DNAstar or DNA club.They were translated to three amino acids sequences respectively.These amino acids were impossible to shape the same three-diamension as BlyS;then they were blasted in protein data bank,it was not found any similarity with B lymphocyte stimulator.So, the possibility that amplified bands were the related sequences to BlyS gene was less. The actions of three sequences need futhur study,which were neither other known genes nor proteins.