Plant Regeneration System And Accumulation Characteristics Of Some Metals In Boussingaultia Gracilis Miers Var. Baselloides Bail.

Boussingaultia gracilis Miers var.baselloides Bail.,belonging to the Basellaceae and Boussingaultia(H.B.K.).It is a common herbs, is also one of research and develop of new resource of wild vegetables in recent years.As to B.gracilis Miers var.baselloides Bail.only blossom not fruit,just we can cuttage and propagate production,is limited seriously in region,to carry out a initial discussion to regenerative system,At the same time,the collection of soil in wild and the B.gracilis Miers var.baselloides Bail.vegetative organs as samples,e.g.As,Ca,Fe,Me,Na,Cd,Mg,Hg,Se,Cr,Cu,K,N,Zn and Pb so on 15 kinds of metal elements to make a analysed,Forcing on Cd and Hg have a strong,enrich ability.We have a research about resistance to abiotic stress,in order to study of mineral nutrition research and promote industrialization to provide some theoretical reference.Choose of B.gracilis Miers var.baselloides Bail.established a regeneration system in each period,added plant growth of regulate assembled and concentration etc.make a discussion.The results stated that the stem with bud induction rate is higher;the MS substrate in proper concentration is 1/ 2MS,Choose 0.2%PVP as a against-brown can prevent brown occurred.In addition,it can't affect induction rate and inducing effects.B.gracilis Miers var. baselloides Bail.in callus induction period,different plants growth regulate to induced the stem with bud callus plays a certain role in promoting,Among them,cytokinins scope for 6-1.5-3.0 BA induces effect is good,the most suitable for 6-BA2.0,in the Auxin, 2,4-D better than NAA,the most suitable is 2,4-D1.0,Plant growth callusl/2MS+6-BA2.0+2,4-d 1.0,induction rate is highest,callus is better.Induce to callus stem,add certain concentration plant growth regulator,improve the induction rate,increase the stem number have a good effect,Among concentration,the 6-BA is 1.5,NAA is 0.5,inducing callus stem has the best effect,induction rate and reach a maximum number of average,and phialide growth is good and balance.Feeding induced clumps root easily,add a low concentration NAA or 2,4-d can promote root differentiation,concentration is 2,4-D1.0,induction rate reached the highest,and the root growth consistency.B.gracilis Miers var.baselloides Bail.vegetative organs as samples,e.g.As,Ca,Fe,Mn,Na,Cd,Mg,Hg,Se,Cr,Cu,K,Ni,Zn and Pb so on 15 kinds of metal elements to make a analysis,results showed that:1) The B.gracilis Miers var.baselloides Bail.tender tip, leaves,buds and beads in various organs such as 15 kinds of metal elements content and accumulation in different organs have a special distinguish.In leaves,K,Ca,Mg,Cd,Mn,Hg,Se,Cu,Zn,and Pb so on,11 kinds of metal elements contents and enrich coefficient are of the highest in four organs,in tenders,As,Fe and Cr,their contents higher than other three organs.All show that cormophyte higher than tuberous.In cormophytes,leaf is higher than cauline, in tuberous,tuber is higher than bulbiet At the same time,the nutrition organs of the Boussingaultia gracilis Miers var. baselloides Bail.the Cd,Hg and K are more strength element enrichment,Fe,Cr and As have only the weakest of enrichment ability.2) Compared with GSCC vegetables,B.gracilis Miers var. baselloides Bail.K,Fe,Mn and Cu,the element contents are higher, the rich of K and Fe make B.gracilis Miers var.baselloides Bail.mineral higher nutrition value,as a new vegetables has certain reference value.At the same time,B.gracilis Miers var. baselloides Bail.in different organs w(K)/w(Na),w(Zn)/w(Cu) and w(Ca)/w(Mg) three-group ratios in clinical research of relatively rational range,to adjust and balance of high blood pressure,coronary heart disease,etc.The corresponding element in population concentration Therefore,the B.gracilis Miers var. baselloides Bail.as functional vegetables to promote.3) To the state of GB/soil environmental quality standard 15618-1995,as evaluation criterior,except for a discrete sample (2),not been polluted in poisonous elements by Hg,As and Pb,has reach the level mostly,which are non-pollution vegetables and herbal plant soil environmental requirements.But in the background,due to the lack of human disturbance,B.gracilis Miers var.baselloides Bail.organs of heavy metal pollution condition so seriously,Among them,Up to "Green Trade Standards of Importing &Exporting Medicinal plants &Preprations",as a result,but for bulbil,other organs had been polluted inordinately by Hg,As and Pb,So,just only bulbil can make medicinal,According to the quality standard of evaluation non-pollution vegetables,four organs had been polluted by Hg,Cd and Pb,in tubers and bulbils are the most badly,so,under natural conditions,so the B.gracilis Miers var.baselloides Bail.we should be carerful to eat,if in the vegetative organs grow.4) B.gracilis Miers var.baselloides Bail.to Cd and Cd-Hg coercion,in a certain extent of toleration,but absorbability and accumulation don't reach the over-enriched plant(Cd and Hg) critical standard;But B.gracilis Miers var.baselloides Bail.has absolute biomass and strong adaptability,etc,it is one type of encrochment plants,we may choose it do a plant to eat when administering soil of Cd and Hg pollution area.