Using Pcr-dgge Analysis Of The Different Habitats Of Phellodendron Amurense Am Fungal Diversity In

This research took root and rhizospheric soil samples of Phellodendron amurense in three different habitats as the research objects. Using traditional morphological method, it can be analyzed the infection situation of arbuscular mycorrhizal fungi of Phellodendron amurense. In this research, molecular biological method was applied to the study in order to analyze the flora composition and the diversity of the AM fungi, which revealed the law of dynamic variation of the AM fungi of Phellodendron amurense in three different habitats. It has important significance to lay the foundation for collection, protection, exploitation of AM fungi resources and for study of functional flora in mycorrhizal of Phellodendron amurense.1. The root samples were investigated by means of acid fuchsin stain after they were lysised in alkaline solution. Then, the mycorrhizal infection rates were calculated. Statistics showed that the root and the AM fungi can form friendly symbiosis. The different infection rate indicated that the infection rate of the mycorrhizal in urban plantation was significantly higher than primary and secondary forests. The infection rate of the mycorrhizal in primeval forest was lowest.2. By determining the physical and chemical properties of soil of Phellodendron amurense in three different habitats, it can be analyzed that the influence of the soil fertility indexes to the infection rate of the mycorrhizal. The average contents of hydrolysable nitrogen and available phosphorus were correlated with the infection rate of AM fungi negatively in three samples. There was no significant correlation between available potassium and the infection rate of AM fungi.3. The techniques of DGGE profiles and Shannon - Weiner diversity index showed that the bands of DGGE profiles existed difference in richness, dominance and diversity about the root and rhizospheric soil samples in different habits. The highest richness and diversity values were the urban plantation of AM fungi in the rhizospheric soil. The richness and diversity values of AM fungi in root were higher than other two areas.4. According to the similarity index (Cs) and UPGMA cluster analysis, the results suggested that AM fungi flora structures of different samples in the same habitat had higher similarity, and in different habitats had lower similarity.5. DGGE band sequences and phylogenetic analysis revealed that all sequences can be divided into four groups of microbial population, which is Glomus, Scutellospora, Diversispora and Hyponectria buxi. There were 37 band sequences of DGGE profile about the root of Phellodendron amurense, in which 34 band sequences had highest similarity to uncultured Glomus. Glomus was the main superiority AM fungi colony in these three habits. And Scutellospora fulgida was detected in all three roots. There was a colony the similarity with Sarcosomataceae of 94%, which didn't belong to AM fungi. It suggested that the specific primers NS31/Glo1 can amplify some colony beyond AM fungi, the specific primers had a certain flaw.