The Research On Mechanism Of Inhabitting Toll-like Receptor4 To Retard The Formation Of Atherosclerosis Through Herbal-cake-separated-moxibustion

Objective:To observe the effect on Toll-like receptor 4(TLR4)mRNA of atherosclerosis rabbits treated by herbal-cake-separated moxibustion and to analyze the possible mechanism.Method:75 rabbits were divided into 5 groups, grouping follows: blank group,model group of AS,direct-moxibustion group,herbal-cake-separated moxibustion group and drug treatment group,15rabbits each group.While fed with high fat food everyday and immune injury except blank group.All these rabbits were feeding for 16weeks. Testing indicators of lipid,the change of ultrastructrre of endothelial cells of the aorta of the rabbits is observed with electron microscope,the expression of TLR4 mRNA.Results:①Compared with model group, the TC and TG content in herbal-cake-separated-moxibustion group,direct-moxibustion group and drug treatment group is obviously lower (P<0.05orP<0.01), Compared with direct-moxibustion group, TC content in herbal-cake-separated-moxibustion group is obviously lower(P<0.01). direct-moxibustion group. herbal-cake-separated moxibustion group and drug treatment group. The LDL content and the ratio of HDL/LDL in three treatment groups is lower than model group(P<0.05 or P<0.01); Herbal-cake-separated-moxibustion group and drug treatment group have the same efficacy (P>0.05); Herbal-cake-separated-moxibustion and drug treatment group have similar regulating effect to the content of apo-A. apo-B, the ratio of A/B (P>0.05).②Under transmission electron microscope, observation of aortic endothelial cells shows:endothelial cells in direct-moxibustion group and herbal-cake-separated-moxibustion group are intacter, most of them are normal in forms, internal elastic membrane is normal, the cells of muscles of medial membrane are relatively normal and the structure of aortic endothelial cells of those groups are similar to those of blank group.The structure of aortic endothelial cells in model group is AS typical lesions, endothelial cells thanatosis, in cytoplasm the sedimentation of a great quantity of lipide can be seen, internal elastic membrane is disruption severely, there is focal dissolution, the cells of smooth muscle in the medial membrane disarragement, and has seriously frothy change.③The expression of TLR4 and TLR4mRNA.in direct-moxibustion group. herbal-cake-separated moxibustion group and drug treatment group is obviously decreased(P<0.05).Conclusion:①Satisfied efficacy on metabolism of lipid,lipoprotein of AS rabbits after treated by direct-moxibustion,herbal-cake-separated moxibustion and drug treatment, herbal-cake-separated moxibustion and drug treatment have the similar effects,both of these method are better than direct-moxibustion.②herbal-cake-separated moxibustion,direct-moxibustion and drug treatment have protective effects on AS rabbit aortic endothelial cells on some contents.③The herbal-cake-separated moxibustion have a certain extent inhibition to the expression of the aortic endothelial cells TLR4.These shows the herbal-cake-separated moxibustion through inhabitting Toll-like receptor4 to retard the formation of Atherosclerosis.