The Affects Of Kangshuaitang On Mitochondrial Dna (mtdna) Of Blood Stasis Syndrome In Rats

Objective Qi blood circulation through the study, sets of square Qingrejiedu law the principle of kangshuai Decoction on line tablets of blood stasis in rat DNA (mtDNA) of the impact, from the molecular level and reveal the mechanism of kangshuai soup for Chinese medicine in anti-the field of prevention and treatment of aging can play a greater role in providing a new theoretical basis.Methods1. To 50 healthy male SD rats were randomly divided into blank control group, Xu Xue card model group, low-dose group, the Dose group, high-dose treatment group of 10 rats.2. Xuxue model group, low, middle and high dose group was given the slow tail vein injection of NE, also began modeling in the first one Days and the first 10 days of tail vein injection of bovine serum albumin BSA, at the same time making model for anti-resistance to prevent blood stasis syndrome Tang, blank control group was given intravenous injection of an equal volume of saline, a continuous 20 days.3. Experiment 20 days after the short-necked rats in each group were sacrificed, followed by extraction of rat liver, heart and brain tissue, using Polymerase Chain Reaction. Shall (hereinafter referred to as PCR), were amplified by wild-type mtDNA, gel electrophoresis, the use of gel imager to scan, depending on display brightness and coverage compare the blank control group and model group, and Xu Xue model group, low-, middle and high-dose treatment group differences in the ratio between the missing.Results1. With the blank control group, model group and low-Xu Xue card in the high-drug group have different levels of mitochondrial deletion(P<0.01).2. Xuxue card with the model group compared with kangshuaitang can significantly reduce the rat liver, heart mtDNA deletion rate (P<0.05). The ratio of brain tissue mtDNA deletion of high-dose kangshuaitang significantly lower (P<0.01). 3. Low, medium and high-dose drug comparison between groups, no significant difference.Conclusions1. PCR products electrophoresis band shown in the aging process, the liver tissue of the fastest aging, mtDNA deletion levels and most Obvious.2. Kangshuaitang can be inhibited to varying degrees in rat liver, heart, brain mtDNA deletion, suggesting that Huayu Qi, heat detoxification of France Prescriptions can reduce oxidative damage to mtDNA, the mtDNA has a protective effect, thus revealing the molecular mechanism of kangshuai tang...