Inhibition Of Proliferation Of Human Cervical Cancer Hela Cells By Casticin In Vitro

BACKGROUD AND PURPOSE Casticin 5, 3′-dihydroxy-3, 6, 7, 4′-tetram thoxyflavone, a polytheoxy flavanoid, CAS,isolated from Vitex tirfolia L, has been reported to inhibit multiple cancer cells proliferation,but there was no report concerning cervical cancer HeLa, this study was aimed to investigate the effect of CAS on inhibition of the growth of human cervical cancer HeLa cells in vitro and part of potential mechanisms.METHODS Human cervical HeLa cells were cultured in vitro and exponentially growing HeLa cells were used in our experiments. The inhibitory effect of CAS on the viability of human cervical cancer HeLa cells was evaluated by the MTT assay. The colony formation was detected by plate colony formation assay. Distribution of cell cycle was analyzed by flow cytometry. The expression of proteins related to cell cycle arrest was analyzed by Western blot.RESULTS CAS significantly inhibited the viability of human cervical cancer HeLa cells in a dose-dependent and time-dependent manner, and the IC50 was 2.82μg/mL for 48h. The colony-forming rate was reduced drastically compared with control group (P<0.05). The cells were markedly accumulated in G2/M phase in a dose-dependent by flow cytometric analysis after treatment with CAS for 48h. Western blot showed that expression of p21 protein was upregulated and protein level of Cyclin B1 was depressed in a concentration dependent after treatment with CAS for 48h.CONCLUSION CAS could inhibit the growth of human cervical cancer HeLa cells in vitro through downregulation of Cyclin B1 expression and upregulatin of p21 protein.