Impact Of Traumatic Shock On Blood Fat And Tnf-α Of Model Rats

Objective: To discuss the impact of traumatic shock on blood fat and TNF-αof rats, and to explore the cholesterol in rats with traumatic shock of contact.Methods:The SD100±20g male rats are randomly divided into two same groups. 30 rats in comparison group are fed with ordinary diet and the 30 rats in experimental group are fed with high-diet group. Their weight are measured periodically and the blood-fat before and after intervention are tests. The traumatic shock model rats are copied and their blood is taken from carotid, which is centrifuged, frozen and stored within 1-4 hours after the shock and before the recovery. Then blood fat and TNF-αis tested. Results: 1 Preparation of animal models: 1) Intervention 43 days later, the comparative group increased TG, but this difference did not change significantly before and after, and TC, LDL-C decreased significantly, HDL-C increased significantly in the comparative group; intervention in the experimental group 43 days after the TG, TC, HDL-C, LDL-C were significantly increased, but increased the level of HDL-C less than the comparative group. Pre-intervention comparative group, experimental group, TG, TC, LDL-C was no significant difference between comparative group, only HDL-C was significantly higher than the experimental group. TG after the intervention of the two groups was no significant difference in the comparative group HDL-C was still significantly higher than the experimental group, which TC, LDL-C was significantly lower than the experimental group.2) After 43 days of intervention, the weight in comparative group and experimental groups is not obviously.2 Traumatic shock to rat blood fats, TNF-αinfluence: 1) Model of traumatic shock during the preparation of the comparative group died 3, the model accounted for 11.1% of the number; the death of the experimental group 8, model accounted for 26.7% of the number. Mortality were unilateral X2 test P = 0.125, both P = 0.186, prompted the two groups was no significant difference in mortality, it can still be seen in the experimental group the control group mortality. Among them, the sudden death of 6, a control group, experimental group 5,The difference has statistics significance. Traumatic shock model into a mold, the exact record of the longest survival time after the recovery of the comparative group 13 hours, and completed the second blood; experimental group four hours, and the rats died after the second blood sampling. 2)The comparative group before and after shock TG, TC, HDL-C, LDL-C significantly, in addition to TG after shock significantly increased, TC, HDL-C, LDL-C significantly reduced; shock in addition to the experimental group significantly increased after the TG , TC, HDL-C, LDL-C is also reduced, but the TC, HDL-C, LDL-C before and after the shock there was no significant difference. Shock before and after the comparative group, experimental group, no significant difference between TG; shock before the comparative group, HDL-C was significantly higher than the experimental group, TC, LDL-C was significantly lower than the experimental group; shock, the same HDL-C was significantly higher than the experimental group, TC, LDL-C was significantly lower than the experimental group. 3) The comparative group during the shock of lower TC was significantly higher than that of the experimental group, TG, HDL-C, LDL-C of the changes in the two groups no significant difference. (Lipid measurement units mmol / L) 4) Control group, after the shock value of TNF-αwas significantly higher than before the shock. Experimental group before and after the shock value of TNF-αis no difference, after the shock than before shock. The comparative group and experimental group during the shock changes in TNF-αvalue of no difference. (TNF-αusing microplate reader reading value) 5) Comparative group, TG increased with the increase of TNF-αwas positively correlated; TC reduction and the reduction of HDL-C was positively correlated; LDL-C lowering and lower HDL-C were negatively correlated; above are related to linear correlation analysis. Experimental group before and after the shock of lower TC and LDL-C reduction was significantly positively correlated, and for the linear correlationConclusion:1 Preparation of animal models: Rats in the growth process of the changes of blood lipids may be the existence of its own anti-atherosclerosis mechanisms result;Animal models with high-fat feed are particularly suitable for pure hypercholesterolemia.2 Under the conditions of traumatic stress in hyperlipidemia, high cholesterol in rats with higher sudden death rate.3 Post-traumatic shock in rats into two groups TG increased the common, ordinary rat TG and the increase in TNF-αsignificantly increased the linear correlation.4 Concerned about cholesterol, the protective effect of the body should not only pay attention when its volume, should also take into consideration the protection of its internal components and damage to components (such as HDL-C/LDL-C) than the composition.5 Pathological state of high cholesterol in rats a higher level there may be a predisposing factor in the role of the outside world to promote cytokine shock (such as IL-1).