| BackgroundTrichomonas vaginalis is a common pathogen in urogeital tract of human,trichomoniasis caused by Trichomonas vaginalis is one of the most common non-virially sexually transmitted diseases(STDs),which accounting for more than 50%of the new cases of STDs.This disease manifests severe clinical symptoms which are closely related to trichomonal virulence and host physiological state.Trichomonas vaginalis usually causes severe urethritists and significantly increases the risk of other sexully transmitted diseases,such as HIV and HBV infection.This risk has been an increasingly important public health concern,so has harm to the human body.Mycoplasma hominis is one of the pathogens that host in the urinary tract and genital tract.As the smallest micro-organism that can live independently,Mycoplasma hominis can cause a variety of symptoms such as spontaneous abortion and postpartum fever and endometrium.T.vaginalis and M.hominis lead to increase the complexity of the pathogenesis. As find of symbiotic relationship between M.hominis and T.vaginalis which both have parasitc life,We believed that it was necessary to re-evaluate their epidemiological genetics and physiological aspects of the mutual.In this case,aiming to know about the information of symbiosis between M.hominis and T.vaginalis and provide a new theoretical basis, Mycoplasma hominis in Trichomonas vaginalis collected from 41 people in Henan province was detected by the means of PCR and specific primer.41 in Henan.T.vaginalis can be naturally infected with intracellular M.hominis,adding yet another genetic dimension in considering all aspects of trichomonas.This paper focuses on relationship between Trichomonas vaginalis and Mycoplasma hominis,found that symbiosis had effects on Genomic polymorphism and molecular evolution of Trichomonas.With the analysis of the genetic polymorphism of T.vaginalis isolates that were gotten from different areas of Henan with the technology of mobile genetic element(MGE)-PCR,the relationship among them was confirmed.According to these relevant elements and the clinical symptoms(symptomatic and asymptomatic) of patients,we hoped to analyze the relationship of these relevant elements and the genetic polymorphism of trichomonas isolates.With the purpose to provide valuable datas for the research on the molecule epidemiology in Henan,the symbiotic relationship between trichomonas and M.hominis were studied.In-depth study of Trichomonas vaginalis and Mycoplasma hominis in a symbiotic relationship will help us better understand the evolution of species to provide a new evolutionary theory.The mechanism of trichomonasis is not entirely clear.Trichomonas infection starts from adhesion host vaginal epithelial cells,this step is essential,this function need surface adhesion protein AP33 of the Trichomonas to complete AP33 gene encoded the surface adhesion protein.AP33 is a very important virulence factor,it adhesions epithelial cell of genitourinary tract of the host by receptors-ligand-mediated.In this papper,PCR amplifying AP33 gene of symptomatical and asymptomatic also mycoplasma symbiotic and mycoplasma-free symbiotic.Analysising sequence,we hope to probe pathogenicity of trichomonas at genetic level.Material and Method41 T.trichomonas were provide by the department of genaecology and obstetrics or test in the affiliated hospital of ZhengZhou University,and all were from secretion and urine of female patients.Developed with Baptist soup,DNA was extracted by the means of phenol-chloroform.Co-existing of M.hominis and T.vaginalis was examined by the means of PCR.Analysising 30 Trichomonas vaginalis with MGE-PCR,we analyzed the length and location polymorphism of gene of Tvmar1 by the means of MGE-PCR among the Trichomonas vaginalis,phylogenetic tree of 30 isolates of Trichomonas vaginalis constructed on basis of MGE-PCR,by the neighbour-joining method and software MEGA4.0. Amplificated AP33gene of 41 trichomonas by PCR,the auther choesd some isolates of T.vaginalis including isolates M.hominis negative and positive and isolates from symptomatical and asymptomatic.In the last,PCR products were sequenced by Biotechnology company of ShangHai.Results1.We detected Myeoplasma hominis in Trichomonas vaginalis by the means of PCR and specific primer.The outcome of PCR detection of the M.hominis in T.vaginalis showed that 34 of 41 T.vaginalis isolates had the special DNA fragment with the length of 334bp, the other 7 failed,with the positive rate of 82.9%.2.Analysis of 41 Trichomonas vaginalis with MGE-PCR showed the genetic distance was between 0 and 100%with the average of 68.05%.The average of similarity coefficient was 31.95%.By the neighbour-joining method and software MEGA4.0,phylogenetic tree of 30 isolates of trichomonas vaginalis showed that 3 groups of T.vaginalis(Tv16,Tv41); (Tv16,Tv37);(Tv37,Tv41) was close.Genetic distance was 0.The result of MGE-PCR showed that the genetic difference was noticeable3.The outcome of AP33 gene showed that all 41 isolates of T.vaginalis had the special DNA fragement with the length of 930bp.By the software of DNASTAR,as a result 8 isolates of T.vaginalis AP33 gene were homology.But to compare and contrast the outcome with the AP33 gene in GenBank(U87098),we found that some bases had changed,sequence similarity among T.vaginalis was as high as 96.8%-99.3%,and sequence difference among T.vaginalis was only as low as 0.7%-3.2%also found the presence of Mycoplasma had a impact on distribution of AP33 gene.in dendrogram.Conclusion1.The Co-existing phenomenon of M.hominis and T.vaginalis that isolated from clinical in defferent areas of Henan is very common.2.Tvmar1 gene of trichomonas vaginalis length polymorphism is not obvious by MGE-PCR and locus polymorphism is an obvious.These differences may be related to the clinical situation co-existing M.hominis but not to area difference.3.AP33 gene of T.vaginalis have scarcely relationship with geographical location clinical manifestations and M.hominis symbiotic... |
PDF Full Text Request