| Objective:To study the intervention effect of classical traditional Chinese medicine prescriptions of Jiangfu Decoction on adriamycin-induced Myocardial Damage.Material and method:Health adult male SD rats were 96,weighting 220～240g,were randomly divided into seven group: the first group is control one (N=11):regular feeding ,no treatment; the second group is model one (N=15):with ADR intraperitioneal injection of adriamycin cardiactoxicity model,2.5mg/kg,week 1,while fed distill water ,1ml/100g, day 1,continuous medication for 4 week; the third group is the third one(N=14): Adriamycin use the same second group,while fed Shengmaiyin (including the amount of liquid 3.5ml/kg/d);the forth group is an attached to an empty group (N=14):with equal physial saline instead of adriamycin,while gavage monkshood decoction (including the amount of crude drug 1.75g/kg/d),drug delivery approach with the model group;the fifth sixth seventh group separated monkshood one (N=14),rhizoma zingiberis one (N=14),Jiangfu one(N=14):use the same model of adriamycin group,were fed at the same time monkshood decoction (including the amount of crude drug 1.75g/kg/d), rhizoma zingiberis group (including the amount of 0.875g/kg/d),Jiangfu group decoction(including the amount of crude drug 2.625g/kg/d),drug delivery approach with the model group. After 4 weeks,rat in each group detected ECG and echocardiography ;the first killed animals and removed heart to calculate the heart index numbler;the second specimens from a part of myocardial specimen were light and electron microscopy to observe the microscopic structure of myocardial tissue and cell ultrastructure changes; the last,we will be made of the remaining myocardial cell homogenate,using chemical colorimetric assay in rat myocardial cells malondialdehyde(MDA) content and superoxide dismutase (SOD),glutethione peroxidase (GSH-Px) activity, a preliminary study intervention Jiang Fu Tang effecting Adriamycin cardiac toxicity in rat.Results:1. General state: model rats, coat wither, listlessness, unresponsive, the lie, lazy to move, slow growth, end the weight, appear hair removal, weight loss phenomenon, a serious stones, ascites, visible diarrhea. In addition to the radix aconiti + model group, the treatment group all appear the model group similar bad state, but came much later, bad state is lighter; And all the radix aconiti + model group bad state a model group appear early, degree of weight.2. Ginger with soup (ADR) of heart toxic injury caused by rat electrocardiogram impact: normal group comparison, model rats, heart rate decreased obviously, QRS complex group of voltage and significantly reduced, Q-T stage was extended, between a statistically significant (P < 0.05); And the model group compared with group, ginger, dry ginger group, radix aconiti + model group of heart rate, QRS complex group of voltage and improved obviously, Q-T stage, and shortens significantly between a statistically significant (P < 0.05); And radix aconiti + group compared to normal, model group of heart rate decreased obviously, QRS complex group of voltage and reduced significantly, and Q-between T stage was extended, radix aconiti + model group more Q-between T stage was extended, which will have a statistically significant (P < 0.05). The comparison between treatment group, dry ginger group of QRS complex group of voltage and Q-T stage between obviously lower than the other two treatment groups, and a statistically significant (P < 0.05).3. Ginger with soup (ADR) of heart toxic injury caused by the rat color Doppler echocardiography influence: model rats, echocardiography change the normal group of EF, FS % declined obviously, and LVIDd, LVIDs increased, which will have a statistically significant (P < 0.05). Group compared with model, radix aconiti + model group, radix aconiti + normal group of LVIDs drop, a statistically significant (P < 0.05).4. Ginger with soup (ADR) of heart toxic injury caused by rat heart heavy influence: group compared with normal, the heart of the model group, a statistically significant weight down (P < 0.05).5. Ginger with soup (ADR) of heart toxic injury caused by rat heart tissue structure influences: light-microscopy, model of rats, myocardial clearance linewidths of muscle fiber, fracture, apparent empty bubble degeneration, particle degeneration, lymphocytic infiltrates phenomenon, and even a qualitative change happened phenomenon. By electron microscopy observation, model rats, myocardial significant damage, muscle fiber fracture, muscle silk disorder, Z line size is differ, partly dissolved, mitochondria swell, cristae vague, and even rupture, individual has appeared sizes of empty bubble degeneration. The treatment group present different degree of myocardial tissue damage, among them, radix aconiti + model group of myocardial cell pathological damage is the most serious.6. Ginger with soup (ADR) of heart toxic injury caused by rat heart muscle cells SOD, GSH-Px, MDA impact: the normal group compared with model rats, myocardial cell SOD, GSH-Px activity declined obviously, myocardial cell MDA content to rise significantly, all have statistically significant (P < 0.05). And the model group compared with group, ginger SOD activity, a significant rise was statistically significant (P < 0.05), GSH-Px activity, MDA content increased by a trend. Comparison between treatment group, ginger attached group SOD activity and dry ginger group, radix aconiti + model group, SOD activity is obviously higher, a statistically significant (P < 0.05).Conclusion:1.Jiangfu decotion to some extent may improve Adriamycin heart toxicity of rats caused by heart function.2. Jiangfu decotion may inhibit doxorubicin toxic rats induced by heart pathological changes of the myocardial tissue structure.3. Jiangfu decotion may reduce Adriamycin heart toxicity through increasing Antioxidant should kinase activity. 4.Jiangfu decotion may product Anti doxorubicin heart toxicity of the effective components/parts that dried ginger or aconite did not possess. |
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