Effect Of Captopril On The Cultivation Of High - Pressure Effect Of Human Umbilical Vein Endothelial Cell Apoptosis

1 Objcctivc Tllere is apparent relatio11ship between vascular endothelialcells aPoptosis and llypertension. The pulPose of this paper was to claritywhether different pressure conditions affect the proliferation andapoptosis of cultured human umbi1ical vein endothelial cells, to observethe protect effects of captopl.i1 on HPC-induced HUVECs apoptosis.2 Mcthods Cells were identified as HUVECs by their growtll pattern andmorphologic features. Experiments were carried out using cel1s from the' 4 th through 6th passages. HUVECs were exposed to either atmosphere,l20, or l80n1mHg pressure by p1acil1g them in a high-tension voltnleterload with 5%co,/air and maintained in RPMI~ l640 supplemented with2.5%ca1f seruln and substrates lbr up to 1 ~ 2 days. \Ve invested thecha1lges of cel1 viability, lnorpho1ogy, agarose gel electrophoresis ofDNA, and flow cytometric a11a1ysis.3 Results The results indicate that HUVECs pro1iferation is influenced bymiddle pressure condition. high pressure condition stimulate proliferationof HUVECs at 16h, however, at 24h and 48h, cel1 11UInber wassigniticant1y lower. Apoptosis cell was markedly increased under highpressul'e conditiOn; "DNA Ladder" was obviously seen at 48h. HoweveT,middle pressure conditiol1 didn't induce apoptosis. An increase in ce1lviabi1ity and a decrease in apoptosis cel1s were observed in captopril (10-'' 10-'mol/L) treated HUVECs by a dose-dependence. "DNA Ladder"disappeared after captopril treatment.4 Conclusions (l ) Harvest and hiaintenance of HUVECs was perfOrmed-by modifled Jaffe's culture method.(2) Ambient pressure in vitro can influence HUVECsgroWth.(3 ) High pressure condition can induce cultured HUVECsapoptosis by a time-dependence.(4 ) Captopril can decrease or inhibit HUVECs apoptosisand reverse groWth inhibition of cultured HUVECsunder high pressure condition.