The Protective Effect Of Polypeptide From Chlamys Farreri On Hela Epithelial Cells And Mouse Skin Ultraviolet Uva Oxidative Damage

AbstractAIM The polypeptides(PCF, Mr:800-1000) was isolated from Chlamys farreri using enzyme engineering technica. We established the oxidative damage models of Hela epithelia cells and hairless mice caused by ultraviolet A to study the protective effects of PCF on Hela epithelia cells and hairless mice damaged by ultraviolet A (cells: UVA3650uJ/cm2, mice: UVA151.68J/cm2). MATHODS The cells were randomly divided into six groups: control group, UVA groups (model, 0.5% PCF, 1% PCF, 2% PCF, 1%VitC). The concentration of GSH-Px, SOD, CAT, MDA, T-AOC and the value of OD were determined by biochemical and MTT methods respectively. The free calcium and the rate of apoptosis and mortality on Hela epithelia cells were tested using flow cytometry (FCM).The mice were randomly divided into five groups: control, UVA (model, 5%PCF, 20%PCF, 10%VitC).The amounts of fibroblasts and were observed by microscope. The expression of Bcl-2 gene and NOS were examined by immunochemical technica. The ultrastructure of skin was observed through electron microscope. RESULTS The results indicated that PCF could enhance the activities of GSH-Px, SOD, CAT, T-AOC and OD values in the cells and the skin tissue. The concentration of cellular free calcium and the amounts of fibroblasts in skin were increased by PCF. The PCF might decrease the amounts of MDA in the cells and the skin tissues and the rate of apoptosis and mortality in Hela epithelia cells. It also could raise the expression of Bcl-2 gene and inhibit the expression of NOS. The ultrastructure of epidermis and fibroblasts of the mice remained normal in PCF groups; cells in epidermis were damaged and there were vacuolations in the smooth endoplasm reticulum in fibroblasts of mice and the number of rough endoplasm reticulum was decreased in UVA model group. The results showed that the observed indications of the PCF groups are significantly different compared with model group (P<0.05). CONCLUSION PCF has the protective effects on oxidative damages of Hela epithelia cells and hairless mice caused by ultraviolet A. Its mechanisms are its abilities of raising the expression of Bcl-2 gene and inhibiting the activity of NOS, increasing anti-oxidative enzymes in cells and skin tissues and inhibiting the of lipid peroxide.