Film Studies, Sodium Hyaluronate To Prevent Post-surgical Adhesions

Post-surgical adhesion formation, a common clinical phenomenon, can cause severe clinical complications. Among such complications are bowel obstruction, infertility, and pain. Occasionally, adhesions necessitate a second operative procedure to remove the adhesion, which may in turn further aggravate the problem. So, seeking the effective way to combat adhesions is an important researching subject in modern surgical area. Recently, much research has been performed in efforts to find ways to inhibit adhesions. These research focuses on coating on the tissue trauma using high molecular materials (such as sodium alginate and chondroitin). But all these haven't achieved clinical recognition just because of the rapid absorption of peritoneum that decease the half-life period of the drugs, non- biodegradation and short duration of the seprafilm peritoneally and lack of safety and so on. A bioresorbable membrane (HA membrane) mainly including sodium hyaluronate was developed to reduce formation of pos-surgical adhesions according to all the above problems. The composition formation of the membrane not only can prevent the formation of adhesions, but also can help to cure.Hyaluronic acid, a kind of acid mucopolysacchride, was first found and separated from vitreous body of cow's eyes by Meyer and Palmer in 1934. Because of it's good biocompatibilit>\ physical (rheological) properties and particular physiological functions, HA has an obvious effect on prevention of post-surgical adhesions. But natural HA hasn't an ideal result to prevent adhesions because of it's short duration at the desired site in vivo. So natural HA is chemically modified in the study and the methods include ion cross-linking and covalent bond cross-linking. Because ion linking has a stronger bound strength than the covalent bond, HA gel modified by ferric chlcride and aluminum chloride has a higher viscoelasticity and was developed into the membrane in the thesis.The technology to develop the cross-linked gel into the membrane was studied and the physical properties were tested in the thesis. Because many property indexes are impossible to be compared in quantity, a comprehensiveassessment system based on stratification is established to appraise the HA membrane. Thus two kinds of membrane-forming agents (sodium carboymethylcellulose and polyvyl alcohol) can be compared and chosen.In the dissertation, the composite formulation of the membrane was studied and optimized through animal experiments. And the synergism between the compositions in the membrane was theoretically analyzed. The optical dosage of compositions in the membrane was achieved through orthogonal design. The animal experiment analytical results show that the factor, TS, has a great diffrential effect on the adhesive grade.