Febrile Disease Damp-heat Wet Weight In The Hot Animal Model Study

Objective:Established the animal model of dampness-heat syndrome more damp with less heat of epidemic febrile disease by simulating clinical pathogeny according to basic theories of TCM. Methods:The animal model of Zhong-Jiao Qi deficiency was first made on the basis of DaHuang spleen-deficiency model. And then put it in the box with high temperature and humidity,and infected by Salmonella Tyhimupium in time. At last,treated it by classic decoction. The test verified if the model was much similar to syndrome more damp with less heat. Result:(1).Symptom simulation:animals under the model fevered,drunk amount decrease,acute swelling of anal region and loose stool. (2).Objective indices:D-xylose excretion rate and amylase activity lowered;SOD lowered and MDA increased,activity of Na+- K+ATPase of erythrocytic membrance lowered significantly,the density of endocellular Na+increased and that of exocellular K+decreased,and ATPase of more damp with less heat group was obviously lower than that of dampness-heat group:Increase of lingual papillae density of lamina propria,and inflammation and hyperemia of lingual papillae;hyperplasia of intestine and stomach mucosa. (3).Classic decoction such as Lei Shi Fang Xiang Hua Zhuo Wan evidently improved and relieved above-mentioned symptoms,signs and indices. Conclusion:The animal model of dampness-heat syndrome more damp with less heat was successfully established. This model exists different indecis changes,which reveal that metablic disorder of materia energy and water may be one of the materia basis of pathophysiology changes of dampness-heat syndrome. At the same time,the decrement of activity of Na+- K4 ATPase and pathology changes of tongue is possibly important indices juging whether dampness of dampness-heat syndrom of epidemic febrile disease is more or less.