| Objective:we attempt to set up a rat model with a syndrome of damp-heat of epidemic febrile disease for beginning, then choose the best animal model, and observe the effect of modified huopuxialing decoction in blood fat, TNF-a, IL-10 and the expression of AQP-5 in tongue tissue of rat model, and investigate the possible mechanism of Chinese herbal compound of clearing damp-heat interventions.Methods:1,80 SPF grade SD rat were divided into ten groups randomly, the normal control group (A),low does pathogenic bacteria group(B,C,D), moderate does pathogenic bacteria group(E,F,G), high does pathogenic bacteria group(H,I,J), under each group establishes 3 different group for different time of damp-heat environment, B,E,H group need 21 days to make model, C,F,I group need 25 days, D,G,J group need 28 days. The model of damp-heat syndrome was established by consulting the method of compound factors, the changes of body temperature,weight,appetite,quantity of drinking,symptom and tongue coating were observed, then analyse the changes of model under differently mold condition, and evaluate the quality of model.2,24 SPF grade SD rat were divided into there groups randomly, the normal control group (natural environment +common fodder),damp-heat syndrome model group (damp and heat environment +fat fodder+ Escherichia coli),medicine interference group (damp and heat environment +fat fodder+ Escherichia coli+ interfered by modified huopuxialing decoction). Making the model of damp-heat syndrome according to the above requirement, and adopt to the condition of 28days and low does pathogenic bacteria. Observing the changes of body temperature,weigh,appete,quantity of drinking,symptom and tongue coating. The level of blood fat were detected by clinical laboratory, the level of in rat serum were detected by ELISA, take the tongue tissue, make pathological sections in normal way, observe the changes of tissue pathology under light microscope, the expression of AQP-5 in rat tongue tissue was analyzed by immunohistochemistry.Results:1,uses the multi-factors to make the model with a syndrome of damp-heat fepidemic febrile disease, every model group rats appears clinical syndrome of damp-heat f epidemic febrile disease in varying degree, regardless in body temperature, appetite, potable water quantity aspect, in the animal symptom which may observed and tongue coating.2,In the aspect of maintaining body temperature's severity and the duration after filling the stomach with bacterium, the pathogenic bacteria low monitoring team (B, C, D group) display well; In the aspect of maintaining mold's low diet quantity and the low potable water quantity,28 days team (D, G, J group) display well; In the aspect of frequency of occurrence of tongue coating and the severity and stable of symptom, 28 days team still to have the obvious superiority; D, I, J group is the best in the Uneven homogeneity of symptom. Quality synthetic evaluation prompts:in the frequency of occurrence,severity,stability and uneven homogeneity of each item of syndrome, D group are good, the model quality is most superior.3,The damp-heat syndrome model group's body temperature,TC,TG,LDL-c level, the blood serum TNF-a and the IL-10 density elevates obviously (P<0.05, P＜0.00);the reduction of body weight,potable water quantity,appetite, and the HDL-c level reduce, compare with the normal control group has the remarkable difference (P<0.05, P<0.00); The medicine intervention group's potable water quantity,appetite obviously increase, the blood serum TNF-α,IL-10 level obviously reduce, compare with the damp-heat syndrome model group has the remarkable difference (P<0.05), compared to normal control group, TNF-a level elevates obviously(P<0.05), other observation target restores, has not remarkable difference (P>0.05)。 4,The damp-heat syndrome model group:cutin cellular layer and prickle cell layer of tongue mucous membrane increased thickness, The incomplete keratinocyte cells increases; midrange lymphocyte,acidophilic cell infiltration, tissue looseness,hydrops were observed in tongue lamina propria, vasocongestion and tissue mild dropsy were observed in muscular layer. The medicine intervention group:cutin cellular layer and prickle cell layer of tongue mucous membrane decreased thickness; compare with the normal control group has not remarkable difference; tongue lamina propria has mild lymphocyte infiltration, muscular layer has no pathological change.5,The integral light density and the area density comparison:compared to normal control group, the AQP-5 expression in tongue tissues obviously increased (P<0.00, P<0.05); Medicine intervention group compare with damp-heat syndrome model group AQP-5 expression remarkable decreased (P <0.05)Conclusion:1,Made the mold under the condition of extending the time of damp-heat environment to 28 days and low dose of intragastrical escherichia coli, may establish satisfied damp-heat syndrome rat model with each symptom obviously, stably and homogeneous.2,The suitable extension of damp-heat environment's time have the positive influence to stability and uneven homogeneity of model rat's symptom, low does escherichia coli may avoided LPS tolerance and rose the body temperature of model rat, highly and stably 3,The damp-heat syndrome model of epidemic febrile disease have lipid disorders, high level of TNF-α,IL-10, pathology changes in tongue tissue and excessive AQP-5 expression. The excessive expression of AQP-5, may relate to the formation of greasy tongue fur.4,Modified huopuxialing decoction can ameliorate model rat's damp-heat syndrome, the mechanism may concerned with the comprehensive accommodation about refrain inflammatory reaction, correct lipid disorders, reduces the tongue tissue inflammation and the AQP-5 expression.
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