Specific Pathways. The Middle And Lower Jiao Yu Mu Points With The Corresponding Organs Fluorescent Double Labeling Study

This subject explores into the nervous connection between back-shu and front-mu points and the corresponding internal organs. Fluorescent double-labeling method was used in the study. The research divides into four parts.The first part is literature review. Through statistics and analysis of ancient and modern literature, it was found that (1) The totalite and variety of the function of channels have determined the complexity of its structure, the function of channels cannot realize by a certain single factor, therefore can not think of its material base too simple. (2) A lot of experimental evidences all prove that the nerve is one of important composition of channels. (3) It is one of the key content of the theory of channels that the acupoints is correlated with internal organs, this is the important theoretical foundation of guiding Chinese medicine to diagnose and treat, it is the tie between channels foundation and clinic, so it is also one of important content of channels study at present. (4) A lot of scholars' researches indicate that the correlation between acupoints and internal organs has close relation with nerve supply. (5) Some researches indicate that the information coming from the body surface and viscera can be assembled at all levels of centers from spinal ganglion to cerebral cortex. (6) Back-shu and front-mu points have extremely close relation with internal organs. No matter ancient time or today doctors always use back-shu and front-mu points to diagnose and treat diseases. It has close relation between the distribution of back-shu and front-mu points and nerve supply. (7) There is little study on "Qijie" at present. The theory of "Qijie" is the foundation of using back-shu and front-mu points. (8)Through the comparison between ancient time and today, it was found that today some back-shu and front-mu points are rarely used at clinic.Therefore the experiment ideas come into my mind, which is to study the nervous connection between back-shu and front-mu points and the corresponding internal organs from morphology point of view. This morphology result is probably the theoretical foundation of using back-shu and front-mu points to treat internal organs diseases, the essence position of "Qijie", the important basis of the relative specificity of the points. It also can explain some channels phenomena.The second part is experiment study. The experiment studies 8 pairs of back-shu and front-mu points and two non-acupoints. The experiment divided into 3 groups: back-shu points --internal organs, front-mu points--internal organs and non-acupoints--stomach. Fluorescent double-labeling method was used in the experiment and use health adult Wister rats to study. PI was separately injected into back-shu points, front-mu points or non-acupoints. The animals survived for 36 hours, and then Bb was pushed into corresponding internal organs. For 12 more hours survival, 4% paraformaldehyde solution in phosphate buffer was perfused through heart, then the DRG were taken, and 40um frozen sections were made for observation. The result is that 6 pairs succeed and other two pairs have some problems. In the same or near Spinal ganglion can observe single-labeling and double-labeling cells. The third part is conclusion. According to the experimental results the following conclusions can be made: (1) The specificity connection path exists between back-shu and front-mu points and corresponding internal organs. These paths are afferent nerves, and assemble at spine ganglion. (2) There exist neurons in the spinal ganglion that control viscera and body surface. This not only provides further explanation to the mechanism of the referred pain, but also indicates that the sensational impulse of acupuncture exert its effect on the functions, activities and sensations of the internal organs through afferent axes at the branches. (3) This study has provided morphological basis for the application of back-shu and front-mu points in the clinical practice. (4) This study also indicates that "Qi...