Hirudin In Vitro Fibroblast Growth Impact

[Objective] to observe the influence of hirudin on the cultured sclera flbroblast and extracellular matrix, and research the mechanism of hirudin treating the diseases caused by the proliferous fibroblast. [Method] cut the rabbit's sclera into 1mm X 1mm, put them into 50 ml culture bottle, add 15% calf serum. To use the third generation flbroblast cells, add DMEM including hirudin 50unite/ml, 40unite/ml, 20unite/ml, lOunite/ml, lunite/ml,the DMEM having not hirudin for compared team, after cultured 48hour later, use the MTT method to detect the absorbing light number(A), collect the liquid, and use the radioimmunoassay to detect the concentration of III collagen and HA.[ Results ] 1. MTT method shows: The hirudin of lOunite/ml has extreme significant difference, contrasting with the compared team(p<0.01), and the arrest rate of cellular growth reached the highest level, the others have not obvious difference ,but the descendent trend still exist2. The concentration of HA of 10 unite/ml is obviously lower than compared team, Having the extreme significant difference (p<0.01), the others have significant difference (p<0.05).3. The concentration of III collagen of 10 unite/ml is obviously lower than compared team, Having the extreme significant difference(p<0.01), the others have significant difference (p<0.05).[Conclusion] Hirudin can repress the proliferation of fibroblast and synthesis and secretion of HA and III collagen , the mechanism may be: restrain fibroblast to synthesize and secrete PDGF, and through inhibiting the action of PDGF on PDGF-R, hold back the proliferation of cell, and restrain fibroblast to synthesize and secrete HA and III collagen.