Experiment Study On Expression Of Matrix Metalloproteinases And Inhibition Of Fibroblasts Of Human Hypertrophic Scar By Hirudin

Hypertrophic scar is the result of excessive repair of post-traumatic organizations after the survival from thermal damage, especially after the healing of deep burns.It is not only affecting the appearance, but also causing local deformity or dysfunction seriously. Currently the proliferating fibroblasts and excessive secretion and deposition of extra cellular matrix are duing to hypertrophic scar. However, the specific formation mechanism is not entirely clear, and there is no effective strategy. Seeking effective measures for prevention and treatment of hypertrophic scar is the main task of the medical workers with trauma research.The treatments of hypertrophic scar by traditional Chinese medicine have broad prospects. The present study shows that a variety of traditional Chinese medicine monomer has a certain inhibitory effect on hypertrophic scars. The study confirms the biological behavior of fibroblasts play an important effect on the process of hypertrophic scar formation with the application of modern molecular biology techniques in the study of mechanism of hypertrophic scar. The proliferation, differentiation and secretion of collagen of fibroblasts are increased after stimulated. Also the expression of matrix metalloproteinases and cytokines is increased. They all involve in the process of wound healing and scar tissue remodeling. It’s known that hirudin has inhibition of hypertrophic scar fibroblasts, but the reports about the effects of hirudin on matrix metalloproteinases are very less. The cell morphology and proliferation activity of fibroblasts and expression of matrix metalloproteinases was observed in this research by different concentrations of hirudin on the planar culture model of hypertrophic scar fibroblasts in vitro. This study is expected to explore the mechanism of hirudin on hypertrophic scar, provide feasibility and argument of hirudin on the treatment of hypertrophic scar in future.Objective To study the effect of hirudin on the proliferation of hypertrophic scar fibroblasts and expression of matrix metalloproteinases, explore the mechanism of hirudin, and provide experimental evidence for clinical medication.Methods10cases of hypertrophic scar tissue were taken from the operated patients who were within6months after injury. Fibroblasts of hypertrophic scar tissue were cultured by tissue block method. The cells after4-7generations were used in experiments, which were interfered with exposure to0U/mL,1U/mL,10U/mL or50U/mL hirudin for24or48hours. We detected the effect of hirudin on the proliferation of fibroblasts by MTT method and the expression of MMP-2, MMP-9by enzyme-linked immunosorbent assay (ELISA).Results①Hirudin could inhibit the proliferation of scar fibroblasts in the groups of1U/mL,10U/mL,50U/mL(P<0.05); the effect of inhibition of hirudin with50U/mL was most significant the inhibition rates of24h,48h were14.75%and15.42%).②The levels of expression of MMP-2and MMP-9were increased with the role of hirudin on fibroblasts after24h, respectively it was most significant with1U/mL and50U/mL. The difference was statistically significant (P<0.05). After48h the expression of MMP-2was decreased, meanwhile the expression of MMP-9was increased but no significance.Conclusion Hirudin can inhibit the proliferation of hypertrophic scar fibroblasts, promote the expression of matrix metalloproteinases (MMP-2, MMP-9) by hypertrophic scar fibroblasts and will help to promote the degradation and reduction of the extracellular matrix.