Chinese Medicine Compound Intestinal Rehabilitation Against Human Colorectal Cancer Role And Mechanism

Objective: To study the anticancer effect of Chinese Compound Recipe Changfukang against humam large intestinal cancer and preliminarily explore its mechanism.Methods: (l)Model rats of transplantation rumor of human large intestinal cancer HT-29 were established. The low, medium and high dose groups of Changfukang ,the blank control group and 5Fu+CF positive control group were set up. (2)After 8 weeks medication of Changfukang through gastrogavage, the antitumor effect of each group was compared. (3)The transplantation tumor karyon morphology, number of apoptotic cells, micro vascular density (MVD) and the gene expression of Ki-67, iNOS, MMP-2 in each group were quantitatively detected and compared by the application of pathomorphology, immunohistochemistry and image analysis technique.Results: (1)Changfukang could significantly inhibit the growth of transplantation tumor, and the tumor's weight in the low, medium and high dose groups was obviously lower than that in the blank group(P<0.05, P<0.01, P<0.001), and that in the 5Fu+CF group (P<0.05). (2)Changfukang could improve the cell and karyon morphology of transplantation tumor, and reduce the physical content in nucleus. This effect was most significant in the high dose group. The Ki-67 count in each Changfukang group was lower than that in the blank group, among which the high dose group had the lowest and the difference was significant compared with the blank group and 5Fu+CF group(P<0.001, P<0.05). (3)The number of apoptotic cells in Changfukang low, medium and high dose groups was separately higher than that in the blank group(P<0.05, P<0.01, P<0.001), and iNOS expression showed a upward tendency, among which the iNOS expression in high dose group was significantly , higher than that in the blankgroup(P<0 01) There was a significant positive correlation between the number of apoptotic cells and the level of iNOS expression in Changfukang treated groups(r = 0.7761, P=0.0001). (4)Changfukang could lower the MVD value of transplantation tumor and the llevel of MMP-2 expresson. And this difference was significant between the high dose group and the blank group(P<0.001, P<0.01).Conclusion: (1)Changfukang has definite antitumor effect against human large intestinal cancer and this effect is dose-dependent .(2)Changfukang has the function of inhibitingthe cell proliferation of human large intestinal cancer, which may be correlated with its effect of improving karyon morphology and reducing the physical anabolism in nucleus. (3)Changfukang has the function of inducing cell apoptosis of human large intestinal cancer, which may be correlated with its promotion of tumor tissue to express high level iNOS. (4)Changfakang has the potential function of anti-invasion and transferation of human large intestinal cancer, which may be correlated with its anti-tumor angiogenesis effect and its inhibition of the degradation of extracellular matrix and basement membrane.