| Stroke is belonged to acute cerebral vascular diseases in modern medicine. The epidemic data collected from various areas all over the world show that acute cerebral vascular diseases are one of the diseases that have the highest mortality. To find effective medicine and therapeutic measures to advance the functional restoration of central nervous system after stroke has always been the emphases of the studies in the interrelated subjects. Studies on protective effect of Chinese traditional medicine have attracted more and more attention in recent years. Buyanghuanwu decoction (BHD) is a classic compound prescription to treat sequelae of stoke. Its protective effect has been validated in clinical practices in several hundred years. In this paper, rats were exposed to reversible middle cerebral artery occlusion without craniectomy, the protective effect of BHD on focal cerebral ischemia-reperfusion injury was studies from biochemical, morphological index and immunohistochemistry results. The paper tries to systematically explain the effect of BHD in order to provide experimental proofs for its clinical practices.Part I : Protective effect of BHD on abnormal behavior and neurological damage of focal cerebral ischemia reperfusion injury in rats40 male Sprague-Dawley rats were randomly divided into: sham group, ischemia-reperfusion group(IR), BHD of a small dose group(10g ?kg"'), BHD of a big dose group(20g kg-1), Nim group. There were 8 animals in each group. BHD 10, 20g kg-1 was directly sent into gastric area for seven days before experiments. The animal models of middle cerebral artery occlusion (MCAO) were established in rats. At 24h after MCAO for 2h, the extent of neurological deficits and cerebral edema as well as the range of cerebral infarction and the changes of pathomorphology in brain tissue of rats was measured. Results showed that BHD could reduced the scores of neurological deficits (P<0. 01, P<0.05), markedly decreased the extent of cerebral edema and the range of cerebral infarction (P<0. 01, P<0. 05), it could significantly reduced the pathological damage of the brain tissue as well.Part II : The possible mechanism of protective effects of BHD on focal cerebral ischemia reperfusion injury in Rats40 male Sprague-Dawley rats were randomly divided into: sham group,ischemia reperfusion group(IR), BHD of a small dose group(10g kg-1), BHD of a big dose group(20g kg-1),Nim group. There were 8 animals in each group. BHD 10, 20g kg-1 was directly sent into gastric area for seven days before experiments. The animal models of middle cerebral artery occlusion (MCAO) were established in rats. At 24h after MCAO for 2h, content changes of malondiadehyde(MDA) and superoxide dismutase (SOD), calcium content and the level of Cerebral Excitatory Amino Acid (EAA) in brain tissue, content changes of endothelin(ET), calcitonin gene related peptide (CGRP) in plasma and nitric oxide (NO) in serum were observed. Results showed BHD could significantly increase SOD activity and decrease the content of MDA in brain tissue (P<0. 01,P<0.05) , the levels of cerebral glutamate(Glu), asparate(Asp) were cut down and the content of calcium in brain tissue were reduced (P<0. 01, P<0. 05) . it could also markedly increase the content of ET, CGRP in plasma and decrease NO in serum (P<0. 01, P<0. 05) .Part III: Effect of BHD on neuron cell apoptosis and expression of apoptosis associated gene of focal cerebral ischemia reperfusion injury in rats.24 male Sprague-Dawley rats were randomly divided into:sham group, ischemia-reperfusion group(IR), BHD group(20g kg-1), The animal models of middle cerebral artery occlusion (MCAO) were established in rats. At 24h after MCAO for 2h, neuron cell apoptosis, the protein expression of Bcl-2 and Bax genes were estimated by electron microscopy and terminal deoxynuleotide transferase (TdT) mediated-dUTP nick end labeling (TUNEL) and immunohistochemistry staining methods respectively. Results showed compared with IR group, the pathological damage of brain tissue attenuated... |
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