The Effects Of Buyanghuanwu Decoction On The Expression Of CyclinD1and CDK2and Cell Proliferation In Rats After Focal Cerebral Ischemia Injury

Objective:To investigate the relationship between the expression of CyclinDl and CDK2and Cell proliferation after focal cerebral ischemia in rats and the intervention effect of BuyangHuanwu Decoction and to explore the neuroprotective mechanism of BuyangHuanwu Decoction from a cell proliferation point and to provide a new understanding of Chinese medicine treatment of cerebral ischemic neuronal damage, provided the experimental basis for its clinical application.Methods:The focal cerebral ischemic model was established by middle cerebral artery occlusion (MCAO), adult rats were randomly divided into four group includeing the normal group,Sham-operated group,ischemia group and BYHWD group,Animals were receiveid different treatment and killed at7,14,21day point after operation.The neurological deficit symptoms were observed and the infarct size ratio was measured by triphenyltetrazolium chloride (TTC).The expression of CyclinDl and CDK2and BrdU-labeled cells were observed by immunohistochemistry technology after the cerebral ischemia.Result:1.Neurological function scores:Normal group, sham group rats were no neurological deficit;compared with the sham group,the model group had seriously neurological deficit at each time point(P<0.01);compared with the model group, BYHWD group could significantly decrease the grade of Neurological function after7,14,21days(P<0.01).BuyangHuanwu Description has a protective effect on cerebral ischemic injury.2.Infarct size ratio:Normal group and sham group were no infarction;compared with the sham group,the model group were observed seriously Cerebral infarction by the TTC staining(P<0.01),infarct size ratio was reduced with the increase of time.Compared with the model group, BYHWD group could effectively reduce infarct size ratio after7,14,21days after cerebral ischemia (P<0.01), infarct size was reduced with the increase in the time of the medication.3.The expression of CyclinD1and CDK2:The normal and Sham group have a small amount of CyclinDl and CDK2;The expression of CyclinDl and CDK2of positive cells increase in the cortex and ischemic penumbra after cerebral ischemia(P<0.01),peaked at7day, with time followed by a decreasing trend. Medication for7days,14days,21days later, BYHWD group could effectively increased the expression of CyclinD1and CDK2in the cortex and ischemic penumbra after cerebral ischemia,compared with the model group(P<0.01),sequentially decreasing trend with time.4.The expression of BrdU positive cell:the normal and Sham group have a small amount of BrdU positive cell, In model group, the expression of BrdU positive cell increase,but not much, The expression increased along the survival time increasing, BYHWD group could significantly increase the expression of BrdU positive cell after7,14days (P<0.01),21days reached the peak. Adjacent sections visible expression of BrdU-positive cells in regional and CyclinDl, CDK2expression area is basically the same.Conclusion:1.Brain tissue has severe neurological damage and cerebral infarction after focal cerebral ischemia due to ischemia, BuyangHuanwu Decoction can reduce ischemic neurological deficit and infarct size.2.Expression of CyclinD1and CDK2increased in Cortical areas and ischemic penumbra after focal cerebral ischemia,BuyangHuanwu Decoction can increase Expression of CyclinD1and CDK2increased in Cortical areas and ischemic penumbra,early effect.3. There is small increase in the expression of BrdU after focal cerebral ischemia,BuyangHuanwu Decoction can significantly increased the expression of BrdU, regulate the expression of CyclinD1, CDK2to increase neuronal proliferation and to induce nerve regeneration, play a role in neuroprotection.