Dried Tangerine Peel In The Anti-tumor Active Ingredient

The effective fraction of Citrus reticulata Bianco are found to have the effect of anti-tumor. The purpose is to study the anti-tumor active ingredient from Citrus reticulata Bianco . The compounds are isolated with silica gel and compounds are identified with UV, MS, 1HNMR,13CNMR spectra. The content of 3,5,6,7,8,3',4'- heptamethphoxyflavone and nobiletin are determined by HPLC.the effects of anti-tumor active ingredient from Citrus reticulata Bianco are extracted and isolated. 10 compounds are isolated with silica gel and compounds are identified with UV, MS, 1HNMR,13CNMR spectra. They are nobiletin , 3,5,6,7,8,3',4'- heptamethphoxyflavone , hesperidin, -sitosterol, 5-hydroxy3',4',6,7,8-hexadicflavone,3,5,6,7, 3',4'- hexadicflavone, glyceric acetate.HPLC figs of the effects of anti-tumor dementia 3,5,6,7,8,3',4'- heptamethphoxyflavone and nobiletin from Citrus reticulata Bianco is established primarily. The result show that the linear range of 3,5,6,7,8,3',4'- heptamethphoxyflavone is 0.025-0.4mg/ml, the correlation coefficients is 1 and the linear range of nobiletin is 0.025-0.4mg/ml, the correlation coefficients is 0.9998. The average recoveries of 3,5,6,7,8,3',4'- heptamethphoxyflavone and nobiletin respectively are 98.74% (n=5,RSD=0:93%), 97.5%(n=5, RSD=2.6%).In a conclusion, Citrus reticulate Bianco has a lot of special flavone. They have certain anti-tumor activity. I have determined the content of 3,5,6,7,8,3',4'- heptamethphoxyflavone and nobiletin from three locations. The result show that the content of 3,5,6,7,8,3 ',4'-heptamethphoxyflavone and nobiletin from Guangzhou are much higher than the other two.